Watanabe Hiroshi, Kaji Chiharu, Anh Dang Duc, Huong Phan Le Thanh, Anh Nguyen Thi Hien, Huong Vu Thi, Phuong Hoang Vu Mai, Thi Ngo Thi, Suu Pham Thi, Nguyet Nguyen Thi Thu, Rusizoka Olivia Sebastian, Watanabe Kiwao, Nagatake Tsuyoshi, Oishi Kazunori
Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
J Clin Microbiol. 2005 May;43(5):2474-6. doi: 10.1128/JCM.43.5.2474-2476.2005.
Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no beta-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns alpha (22 isolates) and beta (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam.
对来自鼻咽拭子(NP)的37株流感嗜血杆菌菌株和来自脑脊液(CSF)的44株流感嗜血杆菌菌株进行了研究。在从NP分离出的37株流感嗜血杆菌中,30株分离株的血清型不可分型,4株为b型,2株为c型,1株为a型,而从CSF分离出的所有44株均为b型。16种抗生素对来自NP和CSF的流感嗜血杆菌分离株的最低抑菌浓度相似,未发现β-内酰胺酶阴性的氨苄西林耐药菌株。通过脉冲场凝胶电泳(PFGE)进行的分子分型显示,来自NP的37株流感嗜血杆菌菌株有22种PFGE图谱,无优势图谱,来自CSF的44株流感嗜血杆菌菌株有9种PFGE图谱,其中α型(22株)和β型(12株)占优势。我们的结果表明,两种主要类型的b型流感嗜血杆菌菌株有可能在越南河内的脑膜炎患儿中传播。