Phan L T Huong, Ngo T Thi, Dang D Anh, Vu T T Huong, Le N Ming, Tran Q Canh, Matsuoka Mayumi, Kamachi Kazunari, Yamazaki Tsutomu, Arakawa Yoshichika, Sasaki Tsuguo
Department of Bacteriology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
Jpn J Infect Dis. 2006 Apr;59(2):111-6.
To investigate Haemophilus influenzae type b (Hib) infection in Vietnamese children under the age of 5 years, cerebrospinal fluid (CSF) samples from patients with meningitis were screened for Hib, and isolates were subjected to evaluation of susceptibility to 12 antibiotics, biotyping, and genotyping with pulsed-field gel electrophoresis (PFGE). The major biotype was type II (68.3%), followed by type I (22.8%). Among 79 Hib isolates, 45 (57%) were beta-lactamase-producing and ampicillin-resistant (44 and 1 isolates produced TEM-1- and ROB-1-type beta-lactamases, respectively), and 34 isolates (43%) were beta-lactamase-nonproducing and ampicillin-sensitive. No beta-lactamase-nonproducing and ampicillin-resistant isolates were found. The PFGE patterns of Hib isolates were highly divergent, but most could be classified into three clusters. We also investigated Hib colonization in household contacts of patients, and found that Hib isolates from the CSF of patients and from nasopharyngeal cavities of household contacts showed the same PFGE patterns. This observation suggested that household contacts of patients are a possible reservoir of Hib.
为调查5岁以下越南儿童的b型流感嗜血杆菌（Hib）感染情况，对脑膜炎患者的脑脊液（CSF）样本进行Hib筛查，并对分离株进行12种抗生素的药敏评估、生物分型以及脉冲场凝胶电泳（PFGE）基因分型。主要生物型为Ⅱ型（68.3%），其次是Ⅰ型（22.8%）。在79株Hib分离株中，45株（57%）产生β-内酰胺酶且对氨苄西林耐药（44株和1株分别产生TEM-1型和ROB-1型β-内酰胺酶），34株（43%）不产生β-内酰胺酶且对氨苄西林敏感。未发现不产生β-内酰胺酶且对氨苄西林耐药的分离株。Hib分离株的PFGE图谱高度不同，但大多数可分为三个簇。我们还调查了患者家庭接触者中的Hib定植情况，发现患者脑脊液和家庭接触者鼻咽腔中的Hib分离株显示相同的PFGE图谱。这一观察结果表明，患者的家庭接触者可能是Hib的一个储存宿主。
