Wu Peter Z J, Zhu Hua, Stapleton Fiona, Hume Emma, Aliwarga Yulina, Thakur Archana, Willcox Mark D P
Vision Cooperative Research Centre and Cornea and Contact Lens Research Unit, School of Optometry and Vision Science, University of New South Wales, Sydney, NSW, Australia.
Curr Eye Res. 2005 Jan;30(1):63-70. doi: 10.1080/02713680490894630.
To examine the role of Staphylococcus aureus alpha-toxin in contact lens-induced peripheral ulceration (CLPU).
Proteolytic enzyme, hyaluronidase, alpha-toxin, and beta-toxin production by S. aureus 8325-4 and its alpha-toxin-deficient mutant (S. aureus DU1090) were examined. Using a rabbit model of CLPU, animals were fitted with hydrogel contact lenses colonized by either S. aureus 8325-4 or the mutant strain. The clinical presentation, bacterial cultures, and histology of the ulceration were examined.
Both strains of S. aureus produced similar levels of caseinase, gelatinase, elastase, hyaluronidase, and beta-toxin. S. aureus DU1090 induced weaker haemolysis of rabbit blood cells than S. aureus 8325-4. Ulceration in the S. aureus DU1090 eye was less frequent and less severe than that caused by S. aureus 8325-4.
The enzyme production profile of S. aureus DU1090 was similar to the parent strain. S. aureus strains may produce CLPU-like lesions irrespective of alpha-toxin production, but severe infectious lesions are produced only in the presence of alpha-toxin.
研究金黄色葡萄球菌α毒素在隐形眼镜诱导的周边溃疡（CLPU）中的作用。
检测金黄色葡萄球菌8325 - 4及其α毒素缺陷突变株（金黄色葡萄球菌DU1090）产生蛋白水解酶、透明质酸酶、α毒素和β毒素的情况。使用CLPU兔模型，给动物佩戴由金黄色葡萄球菌8325 - 4或突变株定植的水凝胶隐形眼镜。检查溃疡的临床表现、细菌培养和组织学情况。
两种金黄色葡萄球菌菌株产生的酪蛋白酶、明胶酶、弹性蛋白酶、透明质酸酶和β毒素水平相似。金黄色葡萄球菌DU1090对兔血细胞的溶血作用比金黄色葡萄球菌8325 - 4弱。金黄色葡萄球菌DU1090引起的眼部溃疡比金黄色葡萄球菌8325 - 4引起的频率更低、程度更轻。
金黄色葡萄球菌DU1090的酶产生情况与亲本菌株相似。金黄色葡萄球菌菌株无论是否产生α毒素都可能产生类似CLPU的病变，但只有在存在α毒素时才会产生严重的感染性病变。
