α毒素缺陷型金黄色葡萄球菌对动物模型周边角膜溃疡形成的影响。

Effects of alpha-toxin-deficient Staphylococcus aureus on the production of peripheral corneal ulceration in an animal model.

作者信息

Wu Peter Z J, Zhu Hua, Stapleton Fiona, Hume Emma, Aliwarga Yulina, Thakur Archana, Willcox Mark D P

机构信息

Vision Cooperative Research Centre and Cornea and Contact Lens Research Unit, School of Optometry and Vision Science, University of New South Wales, Sydney, NSW, Australia.

出版信息

Curr Eye Res. 2005 Jan;30(1):63-70. doi: 10.1080/02713680490894630.

DOI:10.1080/02713680490894630

PMID:15875366

Abstract

PURPOSE

To examine the role of Staphylococcus aureus alpha-toxin in contact lens-induced peripheral ulceration (CLPU).

MATERIALS AND METHODS

Proteolytic enzyme, hyaluronidase, alpha-toxin, and beta-toxin production by S. aureus 8325-4 and its alpha-toxin-deficient mutant (S. aureus DU1090) were examined. Using a rabbit model of CLPU, animals were fitted with hydrogel contact lenses colonized by either S. aureus 8325-4 or the mutant strain. The clinical presentation, bacterial cultures, and histology of the ulceration were examined.

RESULTS

Both strains of S. aureus produced similar levels of caseinase, gelatinase, elastase, hyaluronidase, and beta-toxin. S. aureus DU1090 induced weaker haemolysis of rabbit blood cells than S. aureus 8325-4. Ulceration in the S. aureus DU1090 eye was less frequent and less severe than that caused by S. aureus 8325-4.

CONCLUSIONS

The enzyme production profile of S. aureus DU1090 was similar to the parent strain. S. aureus strains may produce CLPU-like lesions irrespective of alpha-toxin production, but severe infectious lesions are produced only in the presence of alpha-toxin.

摘要