Andrianov Alexander K, Marin Alexander, Roberts Bryan E
Parallel Solutions Inc., 763D Concord Avenue, Cambridge, Massachusetts 02138, USA. aandrianov@ parallelsolutionsinc.com
Biomacromolecules. 2005 May-Jun;6(3):1375-9. doi: 10.1021/bm049329t.
Polyphosphazene polyelectrolytes are potent immunostimulants. Their in vivo performance has been demonstrated for various antigens in a number of animal models. To improve understanding of the mechanism of action, we performed a comparative study in a model system: bovine serum albumin, BSA-poly[di(carboxylatophenoxy)phosphazene], PCPP, in vitro and in vivo. Multi-angle laser light scattering (MALLS) and size-exclusion HPLC methods were used to investigate polyphosphazene-protein formulations in an attempt to establish correlations between their physicochemical behavior and immunostimulating activity. These studies revealed the formation of water-soluble noncovalent protein-polymer complexes in the system. It was shown that both the amount of bound protein and the complex conformation could play an important role in the in vivo performance of the polyphosphazene polyelectrolytes.
聚磷腈聚电解质是强效免疫刺激剂。它们在多种动物模型中针对各种抗原的体内性能已得到证实。为了更好地理解其作用机制，我们在一个模型系统中进行了一项比较研究：牛血清白蛋白、BSA-聚[二（羧基苯氧基）磷腈]（PCPP），包括体外和体内研究。使用多角度激光光散射（MALLS）和尺寸排阻高效液相色谱法来研究聚磷腈-蛋白质制剂，试图建立它们的物理化学行为与免疫刺激活性之间的相关性。这些研究揭示了该系统中形成了水溶性非共价蛋白质-聚合物复合物。结果表明，结合蛋白的量和复合物构象在聚磷腈聚电解质的体内性能中都可能起重要作用。
