Watanabe Junji, Ishihara Kazuhiko
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Biomacromolecules. 2005 May-Jun;6(3):1797-802. doi: 10.1021/bm050138f.
Initial contact between a biological environment and a biomaterial ultimately decides the in vivo performance. Therefore, the fabrication of a delicate biointerface is important because it can be utilized as a platform for novel biomaterials. For the preparation of advanced biomedical devices such as biochips, nanoparticles, and cell engineering devices, the surface properties may be modified by the design of polymeric biomaterials. Anomalous phospholipid polymers with an isomeric oligo(lactic acid) segment were designed and evaluated as a biointerface. The phospholipid polymer containing 2-methacryloyloxyethyl phosphorylcholine was easily copolymerized with isomeric oligo(lactic acid) macromonomers, and the obtained polymer could easily form thin coating membranes as biointerfaces. The oligo(lactic acid) involves three kinds of isomers: dl-, d-, and l-forms. The favorable characteristic on the surface provides regulation of cell-material interactions on the biointerface. The oligo(lactic acid) segment could form hydrophobic domains, which were considered to be located on the interface, to enhance protein adsorption and cell adhesion. The most favorable characteristics on the biointerface were dual functions of cytocompatibility by the phospholipid polymer and cell adhesion property by the oligo(lactic acid) segment. In this study, we focused on the biological responses such as protein adsorption and cell adhesion by change in the oligo(lactic acid) component. The cell viability on the confluent stage was evaluated in terms of metabolic activity.
生物环境与生物材料之间的初始接触最终决定了其体内性能。因此，构建精细的生物界面很重要，因为它可作为新型生物材料的平台。对于生物芯片、纳米颗粒和细胞工程设备等先进生物医学装置的制备，可通过设计聚合物生物材料来改变其表面性质。设计并评估了具有异构低聚乳酸链段的异常磷脂聚合物作为生物界面。含2-甲基丙烯酰氧乙基磷酰胆碱的磷脂聚合物易于与异构低聚乳酸大分子单体共聚，所得聚合物可轻松形成作为生物界面的薄涂层膜。低聚乳酸有三种异构体：dl-型、d-型和l-型。表面的有利特性可调节生物界面上的细胞与材料相互作用。低聚乳酸链段可形成疏水区域，据认为这些区域位于界面上，以增强蛋白质吸附和细胞黏附。生物界面最有利的特性是磷脂聚合物的细胞相容性和低聚乳酸链段的细胞黏附特性这两种功能。在本研究中，我们关注了低聚乳酸成分变化引起的蛋白质吸附和细胞黏附等生物学反应。根据代谢活性评估了汇合阶段的细胞活力。
