Watanabe Junji, Eriguchi Takahisa, Ishihara Kazuhiko
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Biomacromolecules. 2002 Nov-Dec;3(6):1375-83. doi: 10.1021/bm025652p.
Poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA) macromonomers were synthesized for preparation of a novel cytocompatible polymer. The cytocompatible polymer was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and the enantiomeric PLLA (or PDLA) macromonomer. The degree of polymerization of the lactic acid in the PLLA and PDLA segments was designed to be ca. 20. The copolymer-coated surface was analyzed with static contact angle by water. From the result, the PLLA (or PDLA) segment and MPC unit were located on the coated surface, and the monomer unit in the copolymer was reconstructed by contacting water. Fibroblast cell culture was performed to evaluate cell adhesion on the coated surface, and the cell morphology was observed. The number of cell adhesion is correlated with the PL(D)LA content, and the cell morphology is correlated with the MPC unit content. The porous scaffold was prepared by the formation of a stereocomplex between the PLLA and PDLA, and the cell adhesion and following cell intrusion was then evaluated. The fibroblast cells adhered on the surface and intruded into the scaffold through the connecting pores after 24 h. The cell morphology became round shape from spreading with the decreasing PLLA (or PDLA) content in the copolymer. It is considered that the change in the cell morphology would be induced by the MPC unit as cytocompatible unit. These findings suggest that the porous scaffold makes it possible to have cytocompatibility and to produce three-dimensional tissue regeneration.
合成了聚（D-乳酸）（PDLA）和聚（L-乳酸）（PLLA）大分子单体，用于制备一种新型的细胞相容性聚合物。该细胞相容性聚合物由2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）、甲基丙烯酸正丁酯（BMA）和对映体PLLA（或PDLA）大分子单体组成。PLLA和PDLA链段中乳酸的聚合度设计为约20。通过水的静态接触角分析共聚物涂层表面。结果表明，PLLA（或PDLA）链段和MPC单元位于涂层表面，共聚物中的单体单元通过与水接触而重构。进行成纤维细胞培养以评估细胞在涂层表面的粘附情况，并观察细胞形态。细胞粘附数量与PL（D）LA含量相关，细胞形态与MPC单元含量相关。通过PLLA和PDLA之间形成立体复合物制备多孔支架，然后评估细胞粘附和随后的细胞侵入情况。24小时后，成纤维细胞粘附在表面并通过连接孔侵入支架。随着共聚物中PLLA（或PDLA）含量的降低，细胞形态从铺展变为圆形。认为细胞形态的变化是由作为细胞相容性单元的MPC单元诱导的。这些发现表明，多孔支架使得具有细胞相容性并产生三维组织再生成为可能。
