Erickson G F, Magoffin D A, Garzo V G, Cheung A P, Chang R J
Department of Reproductive Medicine, University of California, San Diego, La Jolla 92093-0625.
Hum Reprod. 1992 Mar;7(3):293-9. doi: 10.1093/oxfordjournals.humrep.a137638.
Understanding whether granulosa cells are normal or abnormal in women with polycystic ovary syndrome (PCO) could have clinical importance. For this purpose, we compared the ability of normal and PCO granulosa cells to synthesize oestrogen and progesterone in vitro in response to follicle stimulating hormone (FSH) and/or insulin-like growth factor-I (IGF-I). The normal granulosa cells were from a 7 mm dominant follicle from a women with regular menstrual cycles. The PCO granulosa cells were from 5-7 mm follicles of three patients who had classical PCO. Several interesting points emerge from the comparison: in each PCO patient there was a high level of bioactive FSH in the follicular microenvironment (greater than or equal to 5 mIU/ml; greater than or equal to 250 ng/ml). This is paradoxical because the concentration of steroids in follicular fluid suggests that PCO follicles are highly atretic and therefore should not contain detectable FSH activity. The capacity to secrete progesterone when challenged with a maximally effective dose of FSH and/or IGF-I, was markedly reduced (8- to 10-fold) in PCO compared to normal granulosa cells. This is in sharp contrast to the oestradiol responses which were much the same for PCO and normal granulosa cells. Also, the time course and dose-response effects of FSH showed some major differences between normal and PCO cells, that is, PCO cells lost their capacity to produce oestradiol when treated continuously with a maximally effective dose of FSH. They were also significantly more sensitive to FSH and failed to become more sensitive to IGF-I when treated with FSH.(ABSTRACT TRUNCATED AT 250 WORDS)
了解多囊卵巢综合征（PCO）女性的颗粒细胞是正常还是异常可能具有临床重要性。为此，我们比较了正常和PCO颗粒细胞在体外对促卵泡激素（FSH）和/或胰岛素样生长因子-I（IGF-I）反应时合成雌激素和孕酮的能力。正常颗粒细胞来自月经周期规律女性的一个7毫米优势卵泡。PCO颗粒细胞来自三名患有典型PCO患者的5 - 7毫米卵泡。比较中出现了几个有趣的点：在每个PCO患者的卵泡微环境中都有高水平的生物活性FSH（大于或等于5 mIU/ml；大于或等于250 ng/ml）。这是自相矛盾的，因为卵泡液中的类固醇浓度表明PCO卵泡高度闭锁，因此不应含有可检测到的FSH活性。与正常颗粒细胞相比，当用最大有效剂量的FSH和/或IGF-I刺激时，PCO颗粒细胞分泌孕酮的能力明显降低（8至10倍）。这与PCO和正常颗粒细胞的雌二醇反应形成鲜明对比，二者的雌二醇反应大致相同。此外，FSH的时间进程和剂量反应效应在正常和PCO细胞之间显示出一些主要差异，即当用最大有效剂量的FSH连续处理时，PCO细胞失去了产生雌二醇的能力。它们对FSH也明显更敏感，并且在用FSH处理时对IGF-I没有变得更敏感。（摘要截断于250字）
