Takizawa Toshiaki, Tamiya Masaki, Hara Takuya, Matsumoto Jiro, Saito Naohiro, Kanke Toru, Kawagoe Junichi, Hattori Yukio
Tokyo New Drug Research Laboratories II, Pharmaceutical Division, Kowa Company Ltd., Tokyo 189-0022, Japan.
J Pharmacol Sci. 2005 May;98(1):99-102. doi: 10.1254/jphs.scz050138. Epub 2005 May 7.
Protease-activated receptor 2 (PAR2) belongs to the PAR family (PAR1 to PAR4), which is activated by serine proteases (trypsin, tryptase, etc.). In this study, we evaluated the role of PAR2 in allergic inflammation of airways using PAR2-deficient (PAR2(-/-)) mice. In wild- type mice, infiltration of eosinophils and high eotaxin content were found in bronchoalveolar lavage fluid (BALF) after ovalbumin (OA) sensitization and following challenge. In contrast, both OA-induced infiltration of eosinophils and increase of eotaxin content were abrogated in BALF from PAR2(-/-) mice. The activation of PAR2 might be essential in the production of eotaxin and consequential allergic inflammation in airways.
蛋白酶激活受体2（PAR2）属于PAR家族（PAR1至PAR4），可被丝氨酸蛋白酶（胰蛋白酶、类胰蛋白酶等）激活。在本研究中，我们使用PAR2基因敲除（PAR2(-/-)）小鼠评估了PAR2在气道过敏性炎症中的作用。在野生型小鼠中，卵清蛋白（OA）致敏和激发后，支气管肺泡灌洗液（BALF）中出现嗜酸性粒细胞浸润和高嗜酸性粒细胞趋化因子含量。相比之下，PAR2(-/-)小鼠的BALF中，OA诱导的嗜酸性粒细胞浸润和嗜酸性粒细胞趋化因子含量增加均被消除。PAR2的激活可能在嗜酸性粒细胞趋化因子的产生以及随之而来的气道过敏性炎症中起关键作用。
