Takizawa Mari, Goto Akiko, Watanabe Yuichiro
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo 153-8902, Japan.
Mol Cells. 2005 Apr 30;19(2):228-31.
Recent characterization of several genes involved in plant defense responses suggested that ubiquitin-mediated protein degradation has a role in these responses. We isolated two cDNAs (NtUBA1 and NtUBA2) encoding ubiquitin-activating enzyme (E1) from Nicotiana tabacum cv. BY-2. The open reading frames of both encoded 1080 amino acids, corresponding to molecular masses of 120 kDa. The E1s and corresponding transcripts were upregulated by infection with tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV), and to a lesser extent by cucumber mosaic virus (CMV). Furthermore, they were also upregulated by wounding stress, and the plant hormones salicylic acid, jasmonic acid and the ethylene precursor, aminocyclopropane-1-carboxylic acid (ACC). Our findings support the idea that the ubiquitin-proteasome system plays a role in plant disease defenses.
最近对参与植物防御反应的几个基因的表征表明，泛素介导的蛋白质降解在这些反应中起作用。我们从烟草品种BY-2中分离出两个编码泛素激活酶（E1）的cDNA（NtUBA1和NtUBA2）。两者的开放阅读框均编码1080个氨基酸，对应分子量为120 kDa。E1及其相应的转录本在感染烟草花叶病毒（TMV）和番茄花叶病毒（ToMV）后上调，在较小程度上也受黄瓜花叶病毒（CMV）上调。此外，它们在伤口胁迫以及植物激素水杨酸、茉莉酸和乙烯前体1-氨基环丙烷-1-羧酸（ACC）作用下也上调。我们的研究结果支持泛素-蛋白酶体系统在植物病害防御中起作用这一观点。
