Michetti F, Grilli Caiola M, Botti F, Bertini G, Cocchia D
Istituto di Anatomia Umana, Università di Bari, Italy.
J Histochem Cytochem. 1992 Jun;40(6):839-43. doi: 10.1177/40.6.1588029.
S-100 proteins represent a group of closely related acidic, calcium binding proteins originally isolated from the mammalian nervous system and later detected in non-neural cell types and in a wide variety of vertebrate and invertebrate species. The present study used immunochemical and immunohistochemical methods to extend the investigation of S-100 during phylogenesis to plant tissues. The presence of S-100-like immunoreactive material was detected in extracts of spinach (Spinacia oleracea L.) terminal buds and young leaves by the ELISA method and by Western blotting using different anti-S-100 rabbit antisera. Using the PAP method, serial sections of young spinach leaves treated with the same antisera exhibited an immunoreaction product that was confined to the cytoplasm and nucleus (but absent from the vacuoles) in meristematic, epidermal, and parenchymal cells. The present data enlarge the field of investigation of S-100 proteins in the search of the function(s) of S-100 in biological organisms.
S-100蛋白是一组密切相关的酸性钙结合蛋白，最初从哺乳动物神经系统中分离出来，后来在非神经细胞类型以及多种脊椎动物和无脊椎动物物种中被检测到。本研究采用免疫化学和免疫组织化学方法，将S-100在系统发育过程中的研究扩展到植物组织。通过ELISA法以及使用不同的抗S-100兔抗血清进行蛋白质印迹法，在菠菜（Spinacia oleracea L.）顶芽和幼叶提取物中检测到了S-100样免疫反应性物质。使用PAP法，用相同抗血清处理的菠菜幼叶连续切片显示，免疫反应产物局限于分生组织、表皮和薄壁细胞的细胞质和细胞核中（液泡中没有）。目前的数据拓宽了对S-100蛋白的研究领域，有助于探寻S-100在生物有机体中的功能。
