Liu W G, Zhang J R, Cao Z Q, Xu F Y, Yao K D
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China.
J Mater Sci Mater Med. 2004 Nov;15(11):1199-203. doi: 10.1007/s10856-004-5672-1.
Chitosan (CS) was modified with arginine using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as coupling agents. FTIR and 13C NMR spectra showed that arginine was chemically coupled to CS to form a chitosan-arginine conjugate (CS-ArgC). The substitution degree of arginine in CS estimated from elemental analysis was 20.1%. The circular dichroism spectra indicated that the incorporation of arginine significantly altered the conformation of thrombin; while no obvious variation in the conformation of thrombin was observed with the addition of CS. The anticoagulation activity of glucose aldehyde crosslinked CS-ArgC and CS membranes was evaluated by assaying prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT). The APTT of CS-ArgC membrane was prolonged two times as that of CS counterpart, suggesting that the CS-ArgC is a promising candidate as an anticoagulation biomaterial.
以1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)作为偶联剂，用精氨酸对壳聚糖(CS)进行改性。傅里叶变换红外光谱(FTIR)和碳-13核磁共振光谱(13C NMR)表明，精氨酸通过化学偶联与CS结合，形成壳聚糖-精氨酸共轭物(CS-ArgC)。通过元素分析估算，CS中精氨酸的取代度为20.1%。圆二色光谱表明，精氨酸的掺入显著改变了凝血酶的构象；而添加CS时，未观察到凝血酶构象有明显变化。通过检测凝血酶原时间(PT)、凝血酶时间(TT)和活化部分凝血活酶时间(APTT)，评估了葡萄糖醛酸交联的CS-ArgC和CS膜的抗凝活性。CS-ArgC膜的APTT延长至CS对照膜的两倍，表明CS-ArgC有望成为一种抗凝生物材料。
