Hirano S, Zhang M, Nakagawa M, Miyata T
Chitin/Chitosan R & D, Tottori, Japan.
Biomaterials. 2000 May;21(10):997-1003. doi: 10.1016/s0142-9612(99)00258-6.
Based on an in vitro test for an improvement of the blood compatibility of chitin by blending with tropocollagen, we prepared a novel biocompatible blended fiber and its chemically N-modified fibers. Each (1 g/30 ml) of a clear mixed solution of chitosan with tropocollagen or collagen and a clear solution of chitosan itself in aqueous 2% acetic acid-methanol (2:1, v/v) was spun through a viscose-type spinneret into an aqueous 5% ammonia solution containing 40-43% ammonium sulfate at room temperature to afford a white fiber of chitosan-tropocollagen blends (1.08-1.65 g/denier for the tenacity and 10.9-43.2% for the elongation). The tropocollagen content up to 50% by weight) in the blended fiber affected little their tenacity and elongation values. The blended fiber was chemically N-modified at the fiber state by treatment with a series of carboxylic anhydrides and aldehydes to afford the corresponding N-modified fiber (0.86-1.31 g/denier for the tenacity and 8.0-12.1% for the elongation). A transparent blended hydrogel of N-acetylchitosan (chitin) with tropocollagen was produced from the above mixed solution by treatment with acetic anhydride, and its membrane and sponge sheet were also prepared from the hydrogel.
基于一项通过与原胶原蛋白共混来改善几丁质血液相容性的体外测试，我们制备了一种新型生物相容性共混纤维及其化学 N 改性纤维。将壳聚糖与原胶原蛋白或胶原蛋白的透明混合溶液（各 1 g/30 ml）以及壳聚糖本身在 2%乙酸 - 甲醇（2:1，v/v）水溶液中的透明溶液，通过粘胶型喷丝头在室温下纺丝到含有 40 - 43%硫酸铵的 5%氨水溶液中，得到壳聚糖 - 原胶原蛋白共混的白色纤维（强度为 1.08 - 1.65 g/旦尼尔，伸长率为 10.9 - 43.2%）。共混纤维中原胶原蛋白含量高达 50%（重量）时，对其强度和伸长率值影响不大。通过用一系列羧酸酐和醛处理，在纤维状态下对共混纤维进行化学 N 改性，得到相应的 N 改性纤维（强度为 0.86 - 1.31 g/旦尼尔，伸长率为 8.0 - 12.1%）。由上述混合溶液经乙酸酐处理制得 N - 乙酰壳聚糖（几丁质）与原胶原蛋白的透明共混水凝胶，并由该水凝胶制备了其膜和海绵片。
