Crawford P Cynda, Slater Margaret R, Levy Julie K
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
J Am Vet Med Assoc. 2005 May 1;226(9):1503-7. doi: 10.2460/javma.2005.226.1503.
To determine the sensitivity, specificity, and overall diagnostic accuracy of polymerase chain reaction (PCR) assays offered by commercial diagnostic laboratories for diagnosis of FIV infection in cats.
Prospective clinical trial.
124 cats.
Blood was collected from cats that were neither infected with nor vaccinated against FIV, uninfected cats that were vaccinated with a licensed FIV vaccine, and cats experimentally and naturally infected with FIV representing subtypes A, B, and C. Coded blood samples were submitted to 3 laboratories in the United States and Canada offering PCR assays for diagnosis of FIV infection to veterinary practitioners. All laboratories tested fresh blood samples, and 1 laboratory also tested samples submitted as dried blood smears. The FIV infection status in all cats was confirmed by virus isolation. Sensitivity, specificity, and correct results were calculated for each PCR assay.
Sensitivity ranged from 41% to 93%. Specificity ranged from 81% to 100% in unvaccinated cats and 44% to 95% in cats vaccinated against FIV. Correct results were obtained in 58% to 90% of 124 cats tested. All tests misidentified both uninfected and infected cats. False-positive results by all laboratories were higher in cats vaccinated against FIV than in unvaccinated cats, suggesting that vaccination interferes with the performance or interpretation of PCR assays used for diagnosis of FIV infection.
PCR assays used for diagnosis of FIV infection presently marketed to veterinary practitioners in North America vary significantly in diagnostic accuracy and did not resolve the diagnostic dilemma resulting from vaccination of cats against FIV.
确定商业诊断实验室提供的聚合酶链反应（PCR）检测法对猫免疫缺陷病毒（FIV）感染诊断的敏感性、特异性及总体诊断准确性。
前瞻性临床试验。
124只猫。
采集血液的猫包括既未感染FIV也未接种FIV疫苗的猫、接种了许可的FIV疫苗的未感染猫，以及经实验感染和自然感染FIV（代表A、B和C亚型）的猫。编码后的血样被提交给美国和加拿大的3家为兽医从业者提供FIV感染诊断PCR检测法的实验室。所有实验室均检测新鲜血样，1家实验室还检测了作为干血涂片提交的样本。所有猫的FIV感染状况均通过病毒分离得以确认。计算每种PCR检测法的敏感性、特异性及正确结果。
敏感性范围为41%至93%。未接种疫苗的猫特异性范围为81%至100%，接种FIV疫苗的猫特异性范围为44%至95%。在接受检测的124只猫中，58%至90%的检测获得了正确结果。所有检测均错误识别了未感染和感染的猫。所有实验室的假阳性结果在接种FIV疫苗的猫中高于未接种疫苗的猫，这表明疫苗接种会干扰用于FIV感染诊断的PCR检测法的性能或解读。
目前在北美销售给兽医从业者用于诊断FIV感染的PCR检测法在诊断准确性上差异显著，且未能解决因猫接种FIV疫苗而导致的诊断困境。
