Bienzle D, Reggeti F, Wen X, Little S, Hobson J, Kruth S
Department of Pathobiology, University of Guelph, Guelph, Ontario, N1G 2W.
Can Vet J. 2004 Sep;45(9):753-7.
Diagnosis of feline immunodeficiency virus (FIV) infection by polymerase chain reaction (PCR) has recently become available, but little is known about the performance of this assay. The purpose of this study was to determine the sensitivity and specificity of PCR diagnosis of FIV infection. Replicate aliquots of blood samples from cats identified as FIV positive or negative by 2 previous enzyme-linked immunosorbent assay (ELISA) results, and from clinically healthy dogs, were submitted to different laboratories for FIV serologic diagnosis and PCR. The PCR products obtained in 1 laboratory were sequenced to determine the FIV subtype. The PCR assays correctly identified 100%, 80%, and 50% of the FIV-positive samples, and 100%, 90%, and 70% of FIV-negative samples. Each dog sample was reported as FIV PCR positive at least once, and FIV subtypes A, B, and C were identified. It was concluded that PCR tests currently available for FIV infection are unreliable, with highly variable sensitivity and specificity.
最近，通过聚合酶链反应（PCR）诊断猫免疫缺陷病毒（FIV）感染已经可行，但对于该检测方法的性能了解甚少。本研究的目的是确定PCR诊断FIV感染的敏感性和特异性。将先前通过两种酶联免疫吸附测定（ELISA）结果鉴定为FIV阳性或阴性的猫的血液样本重复等分试样，以及临床健康犬的血液样本，提交给不同实验室进行FIV血清学诊断和PCR检测。对在一个实验室中获得的PCR产物进行测序以确定FIV亚型。PCR检测正确鉴定出100%、80%和50%的FIV阳性样本，以及100%、90%和70%的FIV阴性样本。每个犬样本至少有一次报告为FIV PCR阳性，并且鉴定出了FIV A、B和C亚型。得出的结论是，目前用于FIV感染的PCR检测不可靠，其敏感性和特异性变化很大。
