Zhang Qingguo, Zhao Shifang, Chen Wenbin, Huang Jinglong, Chu Chenglin, Pu Yuepu
Department of Maxillofacial Surgery, Medical College of Zhejiang University, Hangzhou 310006, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Apr;22(2):254-7.
To investigate the ability of composite graft of osteobalsts and deproteinized bone-titanium mesh (DPB-TM) scaffold to repair cranial bone defect. 30 rabbits were randomly divided into 3 groups. The passage 3 fetal rabbit osteoblasts were seeded into porous DPB-TM scaffolds at the density of 5 x 10(6) ml(-1) as the experimental group. The same defects were respectively reconstructed by DPB-TM or osteoblasts as the control groups. After 12 weeks, the result was evaluated by three-dimensional computed tomographic scanning, gross inspection, scanning electron microscopy, histological examination and mechanics test, respectively. In the experimental group, bone trabecula was observed to pass the defect and interface was mixed. No demarcation between the region of the bone defect and the normal bone was observed. There was plenty of new bone on the scaffold. Part of the scaffold was absorbed. In view of mechanics, the intensity of artificial bone (18.93+/-1.12 MPa) was higher than that of normal bone (16.96+/-1.60 MPa) (P<0.05). In the control groups, only fibrous tissue was observed in the defect region, there was no new bone formation. The tissue engineering bone constructed by osteoblasts and DPB-TM scaffold can be applied to the repair of bone defect.
探讨成骨细胞与脱蛋白骨-钛网（DPB-TM）支架复合移植修复颅骨缺损的能力。将30只兔子随机分为3组。将第3代胎兔成骨细胞以5×10(6) ml(-1)的密度接种到多孔DPB-TM支架上作为实验组。分别用DPB-TM或成骨细胞修复相同的缺损作为对照组。12周后，分别通过三维计算机断层扫描、大体观察、扫描电子显微镜、组织学检查和力学测试评估结果。实验组可见骨小梁穿过缺损，界面混合。骨缺损区域与正常骨之间未见分界。支架上有大量新骨形成。部分支架被吸收。力学方面，人工骨强度（18.93±1.12 MPa）高于正常骨（16.96±1.60 MPa）（P<0.05）。对照组缺损区域仅见纤维组织，无新骨形成。成骨细胞与DPB-TM支架构建的组织工程骨可应用于骨缺损修复。
