Hara Arnold H, Jacobsen Christopher M
Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, College of Tropical Agriculture and Human Resources, 461 West Lanikaula Street, Hilo, HI 96720, USA.
J Econ Entomol. 2005 Apr;98(2):284-8. doi: 10.1093/jee/98.2.284.
Mealybug Maconellicoccus hirsutus (Green) adults, nymphs, crawlers, and eggs were tested for their susceptibility to hot water immersion at 47, 48, and 49 degrees C. Eggs inside ovisacs were found most tolerant with prolonged survival compared with other stages at all temperatures. Ovisacs required an average of 1.38, 1.46, and 1.62 times longer treatment duration than adults, nymphs, and crawlers, respectively, for 99.9% predicted mortality at 47, 48, and 49 degrees C. Lethal time estimations were calculated from inverse predictions of regressions derived from logit-transformed data as well as those created using a kinetic model. LT 99.9 estimations were 47.0, 21.2, and 11.9 min at 47, 48, and 49 degrees C, respectively, by using regressions with logit transformations. The kinetic model predictions were 43.9, 19.6, and 11.1 min at 47, 48, and 49 degrees C, respectively. During the study no emergence from eggs inside ovisacs was found after treatments of 52, 24, and 14 min at 47, 48, and 49 degrees C, respectively. Results from this study provide efficacious temperature-time treatments.
对咖啡黑褐软蚧（Maconellicoccus hirsutus (Green)）的成虫、若虫、幼虫和卵进行了47、48和49摄氏度热水浸泡敏感性测试。发现卵囊内的卵在所有温度下与其他虫态相比具有更强的耐受性，存活时间更长。在47、48和49摄氏度下，卵囊达到99.9%预计死亡率所需的处理时间分别比成虫、若虫和幼虫平均长1.38、1.46和1.62倍。致死时间估计值是根据对数转换数据得出的回归反预测以及使用动力学模型得出的回归反预测来计算的。通过对数转换回归分析，在47、48和49摄氏度下，LT99.9估计值分别为47.0、21.2和11.9分钟。动力学模型预测在47、48和49摄氏度下分别为43.9、19.6和11.1分钟。在研究过程中，分别在47、48和49摄氏度下处理52、24和14分钟后，未发现卵囊内的卵孵化。本研究结果提供了有效的温度-时间处理方法。
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