Heuser Michael, Kopun Marijana, Rittgen Werner, Granzow Christof
Abteilung Molekulare Toxikologie (E080), Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
Cancer Lett. 2005 Jun 1;223(1):57-66. doi: 10.1016/j.canlet.2004.10.031. Epub 2004 Dec 8.
A study was performed to improve cytotoxicity determinations by eliminating flavin-mediated photosensitization from tests with KB cells, NCI-H69 cells, P-glycoprotein expressing KBC5-8 cells, MRP1-expressing H69AR cells, and A240286S human lung adenocarcinoma cells. Growth inhibition by cis-platin, doxorubicin, etoposide, gemcitabine, taxol, vincristine, vinblastine, and vinorelbine was determined under flavin-protecting conditions using flavin-free culture media with fetal bovine serum as the only source of flavins. As compared to conventional tests, the IC50 values determined under flavin-protecting conditions reflected increased apparent drug cytotoxicities, and were flawlessly reproducible. Flavin-mediated photosensitization should, therefore, be strictly eliminated from in vitro experiments involving cytotoxic and other drugs.
进行了一项研究，通过在使用KB细胞、NCI-H69细胞、表达P-糖蛋白的KBC5-8细胞、表达MRP1的H69AR细胞和A240286S人肺腺癌细胞的试验中消除黄素介导的光致敏作用，来改进细胞毒性测定。在黄素保护条件下，使用不含黄素的培养基（其中胎牛血清是黄素的唯一来源）测定顺铂、阿霉素、依托泊苷、吉西他滨、紫杉醇、长春新碱、长春碱和长春瑞滨的生长抑制情况。与传统试验相比，在黄素保护条件下测定的IC50值反映出明显的药物细胞毒性增加，并且可完美重现。因此，在涉及细胞毒性药物和其他药物的体外实验中，应严格消除黄素介导的光致敏作用。
