Wang Dong, Weaver Natalie D, Kesarwani Meenu, Dong Xinnian
Developmental, Cell and Molecular Biology Group, Department of Biology, Post Office Box 91000, Duke University, Durham, NC 27708, USA.
Science. 2005 May 13;308(5724):1036-40. doi: 10.1126/science.1108791.
In plants, systemic acquired resistance (SAR) is established as a result of NPR1-regulated expression of pathogenesis-related (PR) genes. Using gene expression profiling in Arabidopsis, we found that in addition to controlling the expression of PR genes, NPR1 also directly controls the expression of the protein secretory pathway genes. Up-regulation of these genes is essential for SAR, because mutations in some of them diminished the secretion of PR proteins (for example, PR1), resulting in reduced resistance. We provide evidence that NPR1 coordinately regulates these secretion-related genes through a previously undescribed cis-element. Activation of this cis-element is controlled by a transcription factor that is translocated into the nucleus upon SAR induction.
在植物中，系统获得性抗性（SAR）是由NPR1调控的病程相关（PR）基因表达所建立的。利用拟南芥中的基因表达谱分析，我们发现，除了控制PR基因的表达外，NPR1还直接控制蛋白质分泌途径基因的表达。这些基因的上调对于SAR至关重要，因为其中一些基因的突变会减少PR蛋白（例如PR1）的分泌，从而导致抗性降低。我们提供的证据表明，NPR1通过一个先前未描述的顺式元件协同调控这些与分泌相关的基因。该顺式元件的激活由一种转录因子控制，该转录因子在SAR诱导后易位到细胞核中。
