瞬时受体电位香草酸亚型8（TRPM8）蛋白在三叉神经节和味蕾中的定位。

TRPM8 protein localization in trigeminal ganglion and taste papillae.

作者信息

Abe Junji, Hosokawa Hiroshi, Okazawa Makoto, Kandachi Mikako, Sawada Yosuke, Yamanaka Keiji, Matsumura Kiyoshi, Kobayashi Shigeo

机构信息

Division of Biological Information, Department of Intelligence Science and Technology, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.

出版信息

Brain Res Mol Brain Res. 2005 May 20;136(1-2):91-8. doi: 10.1016/j.molbrainres.2005.01.013. Epub 2005 Mar 2.

DOI:10.1016/j.molbrainres.2005.01.013

PMID:15893591

Abstract

TRPM8 is a TRP family cation channel which can be activated by cold stimuli or l-menthol. However, TRPM8 protein localization of nerve terminals in sensory organs remains unknown. Here we generated an antibody against TRPM8 and analyzed TRPM8 protein localization in trigeminal ganglia (TG) and in sensory nerve fibers in the tongue. TRPM8 immunoreactivity was detected in a subset of neurons with a small diameter in TG and in nerve fibers in the tongue. TRPM8-immunoreactive nerve fibers were rich in fungiform papillae, but sparse in foliate and circumvallate papillae. The TRPM8-immunoreactive nerve fibers reached the outer epithelial layer in each papilla, while no TRPM8-immunoreactive nerve fibers penetrated into taste buds. Double labeling analysis revealed that TRPM8 immunoreactivity was co-expressed with a part of TRPV1 or CGRP-immunoreactive neurons in TG. However, TRPM8 immunoreactivity was not observed in TRPV1- or CGRP-positive nerve fibers in fungiform, foliate, and circumvallate papillae. These results suggest that TRPM8 protein is present in sensory lingual nerve fibers mainly projected from TG and might work as cold and l-menthol receptors on tongue.

摘要

瞬时受体电位香草酸亚型8（TRPM8）是一种瞬时受体电位（TRP）家族的阳离子通道，可被冷刺激或L-薄荷醇激活。然而，感觉器官中神经末梢的TRPM8蛋白定位仍不清楚。在此，我们制备了一种针对TRPM8的抗体，并分析了三叉神经节（TG）和舌部感觉神经纤维中TRPM8蛋白的定位。在TG中一小部分直径较小的神经元以及舌部的神经纤维中检测到了TRPM8免疫反应性。TRPM8免疫反应性神经纤维在菌状乳头中丰富，但在叶状乳头和轮廓乳头中稀疏。TRPM8免疫反应性神经纤维到达每个乳头的外上皮层，而没有TRPM8免疫反应性神经纤维穿透味蕾。双重标记分析显示，TRPM8免疫反应性与TG中一部分TRPV1或降钙素基因相关肽（CGRP）免疫反应性神经元共表达。然而，在菌状乳头、叶状乳头和轮廓乳头的TRPV1或CGRP阳性神经纤维中未观察到TRPM8免疫反应性。这些结果表明，TRPM8蛋白存在于主要由TG投射的舌感觉神经纤维中，可能作为舌上的冷和L-薄荷醇受体发挥作用。