Bing R J, Dudek R, Kähler J, Narayan K S, Ingram M
Department of Experimental Cardiology, Huntington Medical Research Institutes, Pasadena, CA 91105.
Tissue Cell. 1992;24(2):203-9. doi: 10.1016/0040-8166(92)90093-m.
The release of tumor necrosis factor (TNF), interleukin-1 beta (IL-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) from freshly harvested monocytes and lymphocytes attached to plastic beads was investigated. Previous studies had shown that freshly harvested endothelial cells attached to microcarrier beads release an endothelium-derived relaxing factor. Attachment of freshly harvested lymphocytes and monocytes to plastic beads created a dense network, consisting of 25% monocytes and 75% lymphocytes as shown by flow cytometry. Viability of cells was 90%. Monocytes were characterized by phagocytosis and non-specific esterase stain. Freshly harvested cells stimulated with lipoprotein lipase (LPS) released TNF and IL-1. Non-stimulated cells also produced GM-CSF five hours after collection of blood.
研究了附着于塑料珠上的新鲜收获的单核细胞和淋巴细胞释放肿瘤坏死因子（TNF）、白细胞介素-1β（IL-1）和粒细胞巨噬细胞集落刺激因子（GM-CSF）的情况。先前的研究表明，附着于微载体珠上的新鲜收获的内皮细胞会释放一种内皮源性舒张因子。新鲜收获的淋巴细胞和单核细胞附着于塑料珠上形成了一个密集网络，流式细胞术显示该网络由25%的单核细胞和75%的淋巴细胞组成。细胞活力为90%。单核细胞通过吞噬作用和非特异性酯酶染色进行鉴定。用脂多糖（LPS）刺激新鲜收获的细胞会释放TNF和IL-1。未刺激的细胞在采血后5小时也会产生GM-CSF。
