Essner R, Rhoades K, McBride W H, Morton D L, Economou J S
Division of Surgical Oncology, John Wayne Cancer Clinic, Armand Hammer Laboratories, Los Angeles, California.
J Clin Lab Immunol. 1990 Aug;32(4):161-6.
Human peripheral blood monocytes (PBM) cultured in the presence of 100-5,000 u/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) for 24 hr secreted small quantities of tumor necrosis factor (TNF), but not interleukin-1 (IL-1). The activation of PBM to produce TNF was weak and could be blocked by polyclonal anti-GM-CSF anti-serum. Neither LPS nor IL-2 were synergistic with GM-CSF in the production of TNF or IL-1. IFN gamma alone did not induce either cytokine, but in the presence of GM-CSF it caused a synergistic (100-fold) increase in TNF but not IL-1 production. Macrophage colony-stimulating factor (M-CSF) alone or in combination with LPS, IFN gamma or IL-2 did not stimulate PBM to produce TNF or IL-1 in 24 hr culture.
在100 - 5000单位/毫升粒细胞-巨噬细胞集落刺激因子（GM - CSF）存在的情况下培养24小时的人外周血单核细胞（PBM）分泌少量肿瘤坏死因子（TNF），但不分泌白细胞介素-1（IL - 1）。PBM产生TNF的激活作用较弱，且可被多克隆抗GM - CSF抗血清阻断。在TNF或IL - 1的产生过程中，脂多糖（LPS）和IL - 2均与GM - CSF无协同作用。单独的干扰素γ（IFNγ）不诱导任何一种细胞因子产生，但在GM - CSF存在的情况下，它会使TNF的产生协同增加（100倍），但不会使IL - 1的产生增加。在24小时培养中，单独的巨噬细胞集落刺激因子（M - CSF）或与LPS、IFNγ或IL - 2联合使用均不会刺激PBM产生TNF或IL - 1。
