Kojic Milan, Jovcic Branko, Vindigni Alessandro, Odreman Federico, Venturi Vittorio
Laboratory for Molecular Genetics of Industrial Microorganisms, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11010 Belgrade, Serbia and Montenegro.
FEMS Microbiol Lett. 2005 May 15;246(2):175-81. doi: 10.1016/j.femsle.2005.04.003.
The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N(2-4) GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2D SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.
PsrA转录调节因子参与铜绿假单胞菌中由稳定期诱导的rpoS转录调控以及负向自我调节。本研究旨在确定铜绿假单胞菌中是否有其他基因座受PsrA调控。针对铜绿假单胞菌基因组序列对PsrA结合基序（G/CAAAC N(2 - 4) GTTTG/C）进行计算机搜索。在分析的14个启动子中，有4个对PsrA有反应并与之结合；（i）控制PA2952/PA29 _ 51和PA2953的双向启动子，（ii）PA0506的启动子，以及（iii）PA3571的上游区域。PA0506和PA2952 - PA2951的启动子受到负调控，而PA2953和PA3571的启动子受到PsrA的正调控。还对铜绿假单胞菌PAO1及其psrA基因敲除衍生物的总蛋白进行了二维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（2D SDS - PAGE）分析，结果鉴定出11个差异调节的蛋白斑点。这些研究表明PsrA是一种全局调节因子。
