Sugano Naoyuki, Ikeda Kyoko, Oshikawa Maiko, Idesawa Masataka, Tanaka Hajime, Sato Shuichi, Ito Koichi
Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
J Oral Sci. 2004 Dec;46(4):203-6. doi: 10.2334/josnusd.46.203.
The aim of this study was to assess the relationship-between Porphyromonas gingivalis, Epstein-Barr virus (EBV) infection and reactivation in periodontitis using real-time PCR. The mean proportion of P. gingivalis cells to total bacterial cells in the saliva from EBV-positive periodontitis patients was significantly higher than that in saliva from EBV-negative patients. An EBV-positive B-cell line was used to determine whether P. gingivalis sonicate induced reactivation of EBV, using real-time PCR to measure the virus genome in the culture medium. A significant increase in EBV numbers was observed after the stimulation with P. gingivalis sonicate. These findings suggest that the interaction between EBV and P. gingivalis is bi-directional, with EBV reactivation suppressing host defenses and permitting overgrowth of P. gingivalis, and P. gingivalis having the potential to induce EBV reactivation.
本研究的目的是使用实时聚合酶链反应（PCR）评估牙龈卟啉单胞菌、爱泼斯坦-巴尔病毒（EBV）感染与牙周炎再激活之间的关系。EBV阳性牙周炎患者唾液中牙龈卟啉单胞菌细胞占总细菌细胞的平均比例显著高于EBV阴性患者唾液中的比例。使用EBV阳性B细胞系，通过实时PCR测量培养基中的病毒基因组，以确定牙龈卟啉单胞菌超声裂解物是否诱导EBV再激活。用牙龈卟啉单胞菌超声裂解物刺激后，观察到EBV数量显著增加。这些发现表明，EBV与牙龈卟啉单胞菌之间的相互作用是双向的，EBV再激活抑制宿主防御并使牙龈卟啉单胞菌过度生长，而牙龈卟啉单胞菌有可能诱导EBV再激活。
