基于RAPD分析的满江红鱼腥藻及其宿主植物（满江红）的遗传多样性与同源性

[The genetic diversity and homology of Anabaena azollae and its host plant (Azolla) based on rapd analysis].

作者信息

Chen Jian, Zheng Wei-wen, Xu Guo-zhong, Song Tie-ying, Tang Long-fei

机构信息

Azolla Research Center, Fujian Academy of Agricultural Sciences[FAAS], Fuzhou 350013, China.

出版信息

Yi Chuan. 2002 Jan;24(1):45-9.

PMID:15901562

Abstract

Symbiotic Anabeana azollae and its host plant Anabeana-free Azolla were isolated from 16 Azolla accessions representing different Azolla species or geographic origins.DNA polymorphic fragments were obtained by simultaneous RAPD amplification of both symbiont and host. The UPGMA clusters of Anabeana azollae and its host Azolla were established separately based on Dice coefficient caculation and a coordinated relationship was shown between Anabeana azollae and its Azolla host along both individual genetic divergence,but this genetic homology was reduced among different strains within Azolla species while the obvious mutants of Anabeana azollae were detected in some Azolla tested strains collected from different geographic area in the same host species.

摘要

共生的满江红鱼腥藻及其宿主植物无鱼腥藻满江红是从代表不同满江红物种或地理来源的16个满江红种质中分离出来的。通过对共生体和宿主同时进行随机扩增多态性DNA（RAPD）扩增获得了DNA多态性片段。基于迪西系数计算分别建立了满江红鱼腥藻及其宿主满江红的非加权组平均法（UPGMA）聚类，结果表明满江红鱼腥藻与其满江红宿主在个体遗传差异上存在协同关系，但在满江红物种内不同菌株间这种遗传同源性降低，同时在从同一宿主物种不同地理区域收集的一些满江红测试菌株中检测到了满江红鱼腥藻的明显突变体。