Croxton T L, Armstrong W M
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202-5120.
Am J Physiol. 1992 May;262(5 Pt 1):C1324-34. doi: 10.1152/ajpcell.1992.262.5.C1324.
Measurements of intracellular and extracellular ion activities with ion-selective microelectrodes generally involve calibration of the electrodes in solutions of known composition and fitting of the calibration data with a theoretical expression. The Nicolsky equation is frequently used to describe the variation of electrode potential with primary ion activity in the presence of a constant amount of interference. In this report, we review the estimation of primary ion activities in calibration solutions and discuss the practical use of the Nicolsky equation. We describe a specialized computer program, developed in this laboratory, for routine input and editing of calibration data, fitting of data with the Nicolsky equation, and calculation of experimental ion activities from the fitted curve. Earlier versions of this program have proven helpful in several investigations in this and in other laboratories.
使用离子选择性微电极测量细胞内和细胞外离子活性，通常需要在已知成分的溶液中对电极进行校准，并将校准数据与理论表达式进行拟合。在存在恒定干扰量的情况下，Nicolsky方程经常用于描述电极电位随主要离子活性的变化。在本报告中，我们回顾了校准溶液中主要离子活性的估计方法，并讨论了Nicolsky方程的实际应用。我们描述了一个在本实验室开发的专门计算机程序，用于校准数据的常规输入和编辑、数据与Nicolsky方程的拟合以及根据拟合曲线计算实验离子活性。该程序的早期版本已在本实验室和其他实验室的多项研究中证明是有用的。
