Rout U K
Division of Science and Technology, Griffith University, Brisbane, Australia.
Alcohol Clin Exp Res. 1992 Apr;16(2):286-9. doi: 10.1111/j.1530-0277.1992.tb01378.x.
Alcohol dehydrogenase (ADH) phenotypes were investigated in the brain of 15 different inbred mice by isoelectric focusing followed by staining of enzyme activities. The Class III ADH activity was detected in all the strains studied, whereas the Class II ADH activity was found only in few strains (including the alcohol-preferring strain--C57BL/6J) having the "a" allele (ADH-C2(2)) for this isozyme in stomach. The inbred strains having the "b" allele (ADH-C2(1)) for the Class II ADH in stomach (including the alcohol avoiding strains--BALB/c, CBA/H, C3H/He, DBA/2J, and SJL/J) demonstrated null variant for this phenotype in their brain. The Class I ADH activity was very low or absent in the brain extracts of all the strains studied. The ADH activities were confined to the cytosolic fractions of brain and were higher in the extracts of cerebral hemispheres than in cerebellum. The genetic linkage studies showed that the locus for the brain Class II ADH is closely linked to the "Adh gene complex" on chromosome 3 of mice.
通过等电聚焦随后对酶活性进行染色，研究了15种不同近交系小鼠大脑中的乙醇脱氢酶（ADH）表型。在所研究的所有品系中均检测到Ⅲ类ADH活性，而Ⅱ类ADH活性仅在少数品系（包括嗜酒品系——C57BL/6J）中发现，这些品系在胃中具有该同工酶的“a”等位基因（ADH-C2(2)）。在胃中具有Ⅱ类ADH的“b”等位基因（ADH-C2(1)）的近交系（包括避酒品系——BALB/c、CBA/H、C3H/He、DBA/2J和SJL/J）在其大脑中表现出该表型的无效变异。在所研究的所有品系的脑提取物中，Ⅰ类ADH活性非常低或不存在。ADH活性局限于大脑的胞质部分，且大脑半球提取物中的活性高于小脑。遗传连锁研究表明，大脑Ⅱ类ADH的基因座与小鼠3号染色体上的“Adh基因复合体”紧密连锁。
