抗原激发时血小板活化因子的释放。一种研究药物过敏反应的方法。

PAF acether release on antigenic challenge. A method for the investigation of drug allergic reactions.

作者信息

Dunoyer-Geindre S, Ludi F, Perez T, Terki N, Gumowski P I, Girard J P

机构信息

University Cantonal Hospital of Geneva, Switzerland.

出版信息

Allergy. 1992 Feb;47(1):50-4. doi: 10.1111/j.1398-9995.1992.tb02249.x.

DOI:10.1111/j.1398-9995.1992.tb02249.x

PMID:1590567

Abstract

This study describes an in vitro method to evaluate a PAF acether release test (PART) from white blood cells after antigenic challenge. PAF acether activity of the supernatant was tested by platelet aggregation. The aggregating power was abolished by using SRI 63-441 (Sandoz), a PAF acether inhibitor. This method was applied to 57 patients with allergic or pseudo-allergic reactions to drugs by using different drug protein conjugates. The results of PART were evaluated in relation to the clinical history (score of imputability) and to other tests (skin tests, lymphocyte transformation tests (LTT), IgE-RAST). A good correlation was found between the release of PAF acether and a high predictability score: sensitivity 75%, specificity 83.8%. PART also correlated with skin tests (75% agreement, n = 60), with LTT (67.7% agreement, n = 74) and in 65.6% of cases with positive penicillin IgE-RAST (n = 32). This method brings a new possibility for the investigation of drug-allergic and pseudo-allergic reactions.

摘要

本研究描述了一种体外方法，用于评估抗原刺激后白细胞的血小板活化因子乙酰醚释放试验（PART）。通过血小板聚集试验检测上清液中血小板活化因子乙酰醚的活性。使用血小板活化因子乙酰醚抑制剂SRI 63 - 441（山德士公司）可消除聚集能力。通过使用不同的药物蛋白偶联物，将该方法应用于57例对药物有过敏或类过敏反应的患者。根据临床病史（可归因性评分）和其他检查（皮肤试验、淋巴细胞转化试验（LTT）、IgE - RAST）对PART的结果进行评估。发现血小板活化因子乙酰醚的释放与高预测性评分之间具有良好的相关性：敏感性为75%，特异性为83.8%。PART还与皮肤试验（一致性为75%，n = 60）、LTT（一致性为67.7%，n = 74）以及65.6%的青霉素IgE - RAST阳性病例（n = 32）相关。该方法为药物过敏和类过敏反应的研究带来了新的可能性。