Koller D Y, Pirker C, Jarisch R, Götz M
Dermatologic and Pediatric Allergy Clinic, Vienna, Austria.
Allergy. 1992 Feb;47(1):58-9. doi: 10.1111/j.1398-9995.1992.tb02251.x.
We investigated the effect of the histamine control (1 mg/ml) on the results of skin prick and intradermal testing with bee and wasp venom. Skin tests were done on the patients' forearms: on the right arm the histamine control and the bee venom dilutions, on the left arm the wasp venom dilutions only, at distances of 4-5 cm. In intradermal testing 11 (9%) of 122 patients showed a positive wheal and flare reaction to the bee venom solution positioned next to the histamine control. The subsequent solutions in higher concentrations did not produce any skin reactions. The results of intradermal testing with bee venom did not occur in intradermal testing with wasp venom or in skin prick testing with both allergens. Our results show clearly that in skin prick tests a distance of 4-5 cm is sufficient to avoid false positive skin reactions. However, using the same distance in intradermal testing showed that histamine affects the skin reactions produced by adjacent allergen solutions. Therefore false positive results may occur.
我们研究了组胺对照品（1毫克/毫升）对蜜蜂和黄蜂毒液皮肤点刺试验及皮内试验结果的影响。皮肤试验在患者前臂进行：右臂进行组胺对照品和蜜蜂毒液稀释液试验，左臂仅进行黄蜂毒液稀释液试验，试验点间距4 - 5厘米。在皮内试验中，122例患者中有11例（9%）对紧邻组胺对照品的蜜蜂毒液溶液出现阳性风团及潮红反应。后续更高浓度的溶液未引起任何皮肤反应。用黄蜂毒液进行皮内试验以及用这两种变应原进行皮肤点刺试验时，均未出现用蜜蜂毒液进行皮内试验的结果。我们的结果清楚表明，在皮肤点刺试验中，4 - 5厘米的间距足以避免假阳性皮肤反应。然而，在皮内试验中使用相同间距时发现，组胺会影响相邻变应原溶液产生的皮肤反应。因此可能会出现假阳性结果。
