芥子碱的电化学性质及其在药用植物中的检测

Electrochemistry of sinapine and its detection in medicinal plants.

作者信息

Zhou Hui, Huang Yinxi, Hoshi Tomonori, Kashiwagi Yoshitomo, Anzai Jun-Ichi, Li Genxi

机构信息

Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.

出版信息

Anal Bioanal Chem. 2005 Jun;382(4):1196-201. doi: 10.1007/s00216-005-3241-1. Epub 2005 May 20.

DOI:10.1007/s00216-005-3241-1

PMID:15906009

Abstract

Sinapine (O-sinapoyl choline) is a crucial component, with much medicinal value, of many dietary and medicinal plants. It has been found that sinapine gives an electrochemical response at a pyrolytic graphite electrode. The electrochemical properties of sinapine have been investigated. The peak current in the cyclic voltammogram is linear in the concentration range 1.9 x 10(-6)-2.5 x 10(-4) mol L(-1) and the limit of detection is 9.9 x 10(-7) mol L(-1). These properties can be applied to the determination of sinapine in extracts from three kinds of medicinal plant. The electrochemical method reported here is highly selective, sensitive, and stable.

摘要

芥子碱（O-芥子酰胆碱）是许多食用和药用植物的关键成分，具有很高的药用价值。研究发现，芥子碱在热解石墨电极上会产生电化学响应。已对芥子碱的电化学性质进行了研究。循环伏安图中的峰值电流在1.9×10⁻⁶ - 2.5×10⁻⁴ mol L⁻¹浓度范围内呈线性，检测限为9.9×10⁻⁷ mol L⁻¹。这些性质可应用于三种药用植物提取物中芥子碱的测定。本文报道的电化学方法具有高选择性、高灵敏度和稳定性。