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骚扰阿蚊唾液腺蛋白质分析

Analysis of salivary gland proteins of the mosquito Armigeres subalbatus.

作者信息

Siriyasatien Padet, Tangthongchaiwiriya Kuntida, Jariyapan Narisara, Kaewsaitiam Sakchai, Poovorawan Yong, Thavara Usavadee

机构信息

Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 2005 Jan;36(1):64-7.

Abstract

Quantitative studies of total salivary gland protein of Armigeres subalbatus mosquito revealed that the total salivary gland protein increased dramatically during the five days after emergence as adults. The amount of salivary gland protein of female and male mosquitos at day five after adult emergence were on the average 11.55 and 1.32 microg/pair gland respectively. SDS-PAGE studies showed that salivary gland protein profiles of Armigeres subalbatus demonstrated 9 major polypeptide bands of 68, 65, 60, 55, 40, 30, 28, 21, and 15 kDa. The 21 and 65 kDa bands were found only in the distal lateral region of the mosquito salivary gland and were depleted after the female mosquito took a blood meal.

摘要

对骚扰阿蚊唾液腺总蛋白的定量研究表明,成虫羽化后的五天内唾液腺总蛋白急剧增加。成虫羽化后第五天,雌蚊和雄蚊唾液腺蛋白量平均分别为11.55和1.32微克/对腺体。SDS-PAGE研究表明,骚扰阿蚊的唾液腺蛋白谱显示出9条主要多肽带,分子量分别为68、65、60、55、40、30、28、21和15 kDa。21 kDa和65 kDa的条带仅在蚊唾液腺的远外侧区域发现,且雌蚊吸血后会减少。

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