Guo X, Beerntsen B T, Zhao X, Christensen B M
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
J Parasitol. 1995 Apr;81(2):200-7.
The involvement of hemocytes in melanotic encapsulation reactions against Brugia malayi was assessed in Armigeres subalbatus. Hemocyte populations, epitope changes, phenol oxidase (PO) activity, and the presence of an 84-kDa polypeptide were investigated in mosquitoes exposed to a B. malayi-infective bloodmeal (= immune-activated), in mosquitoes given a noninfective bloodmeal (= controls), in nonbloodfed mosquitoes (= naive), or in some combination of these. Total hemocyte populations in immune-activated mosquitoes significantly decreased at 24 hr postbloodmeal (PB) as compared with controls. At 48 and 72 hr PB, hemocyte population levels in immune-activated mosquitoes increased to control levels. Epitope changes, as indicated by wheat germ agglutinin (WGA) binding, also were observed. There was a significant increase in the percentage of hemocytes binding WGA in immune-activated mosquitoes at 24 hr PB as compared with controls. Furthermore, the activity of hemocyte PO, an enzyme involved in the melanotic encapsulation pathway, was significantly elevated at 12 hr PB in immune-activated mosquitoes as compared with controls. Analysis for the presence of an 84-kDa polypeptide in A. subalbatus indicates that a 2.0-kb message in total RNA hybridized to D6.12, an Aedes aegypti cDNA encoding an 84-kDa polypeptide that is associated with melanotic encapsulation responses. The hybridization of D6.12 to RNA was not greater in immune-activated as compared to control A. subalbatus, as has been observed in A. aegypti. Results indicate that these hemocyte changes correspond in time with the melanotic encapsulation reactions of A. subalbatus against filarial worms.
在骚扰阿蚊中评估了血细胞参与针对马来布鲁线虫的黑化包囊反应的情况。研究了暴露于感染马来布鲁线虫的血餐（即免疫激活）的蚊子、给予非感染性血餐的蚊子（即对照）、未吸血的蚊子（即未致敏）或这些情况的某种组合中的血细胞群体、表位变化、酚氧化酶（PO）活性以及一种84 kDa多肽的存在情况。与对照相比，免疫激活的蚊子在血餐后24小时总血细胞群体显著减少。在血餐后48小时和72小时，免疫激活的蚊子中的血细胞群体水平增加到对照水平。还观察到了如小麦胚凝集素（WGA）结合所表明的表位变化。与对照相比，免疫激活的蚊子在血餐后24小时结合WGA的血细胞百分比显著增加。此外，与对照相比，免疫激活的蚊子在血餐后12小时参与黑化包囊途径的血细胞PO活性显著升高。对骚扰阿蚊中84 kDa多肽存在情况的分析表明，总RNA中的一个2.0 kb信息与D6.12杂交，D6.12是埃及伊蚊的一个编码与黑化包囊反应相关的84 kDa多肽的cDNA。与埃及伊蚊中观察到的情况不同。与对照骚扰阿蚊相比，免疫激活的阿蚊中D6.1至RNA的杂交并不更强。结果表明，这些血细胞变化在时间上与骚扰阿蚊针对丝虫的黑化包囊反应相对应。
