Mlinaric Ales, Kac Javor, Pohleven Franc
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Slovenia.
Acta Pharm. 2005 Mar;55(1):69-79.
Extracts obtained using methanol and dichloromethane from 57 species of wood damaging fungi were investigated for their ability to inhibit HIV-1 reverse transcriptase activity in vitro using a non-radioactive assay. Sixty-three samples were tested all together; some species were represented more than one isolate. Thirteen methanolic extracts exhibited more than 40% inhibition and two among them inhibited the enzyme by more than 80%. All extracts obtained with dichloromethane were inferior to methanolic extracts in their inhibitory activity. The most active fungal species discovered in the first screening were Laetiporus sulphureus and Poria monticola, followed by Poria vaillanti and Chondrostereum purpureum. In the second screening, Laetiporus sulphureus was selected for detailed examination and different isolates were tested. Preliminary findings confirmed the presence of an acidic compound with the amino group in the most active fraction.
使用甲醇和二氯甲烷从57种木材腐朽真菌中提取的提取物，采用非放射性检测方法对其体外抑制HIV-1逆转录酶活性的能力进行了研究。总共测试了63个样品；有些物种有不止一个分离株。13种甲醇提取物表现出超过40%的抑制作用，其中两种对该酶的抑制作用超过80%。所有用二氯甲烷获得的提取物在抑制活性方面均不如甲醇提取物。首次筛选中发现的活性最高的真菌物种是硫黄多孔菌和蒙地卧孔菌，其次是瓦氏卧孔菌和紫孢块菌。在第二次筛选中，选择硫黄多孔菌进行详细检测，并对不同的分离株进行了测试。初步研究结果证实，活性最高的组分中存在一种含氨基的酸性化合物。
