Lo Shyh-Ching, Wang Richard Y-H, Grandinetti Teresa, Zou Nianxiang, Hayes Michael M, Shih Jim W-K, Wear Douglas J
Department of Environmental and Infectious Disease Sciences, Armed Forces Institute of Pathology, Building 54, Room 4091, 14th Street and Alaska Avenue NW, Washington, DC 20306, USA.
FEMS Immunol Med Microbiol. 2005 Jun 1;44(3):277-82. doi: 10.1016/j.femsim.2004.12.010.
We examined Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to M. penetrans isolation reacted with a number of M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of M. penetrans with molecular mass of 35 and 38 kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for M. penetrans infection.
我们检测了5名男性同性恋艾滋病患者血清中的穿透支原体特异性抗体，这些患者在病程中分离出了穿透支原体。使用全细胞蛋白进行蛋白质印迹分析时，未发现一致的免疫反应模式。在分离出穿透支原体之前采集的血清样本与多种穿透支原体蛋白发生反应，这很可能是由于非特异性交叉反应所致。进一步分析发现，患者在分离出支原体时对穿透支原体的脂质相关膜蛋白（LAMPs）产生了显著的抗体反应，而在分离出穿透支原体之前采集的血清样本中未观察到这种反应。阳性抗体反应主要针对分子量为35 kDa和38 kDa的两种主要穿透支原体LAMPs，并产生了独特的阳性免疫反应条带模式。我们的观察结果表明，与整个支原体蛋白相比，LAMPs在穿透支原体感染的血清学检测中是更具特异性的靶抗原。
