来自巴西亚马逊地区临床环境的铜绿假单胞菌分离株中的1类整合子。
Class 1 integrons in Pseudomonas aeruginosa isolates from clinical settings in Amazon region, Brazil.
作者信息
Fonseca Erica L, Vieira Verônica V, Cipriano Rosângela, Vicente Ana C P
机构信息
Department of Genetics, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brazil 4365, P.O. Box 926, CEP 21045-100, Rio de Janeiro, Brazil.
出版信息
FEMS Immunol Med Microbiol. 2005 Jun 1;44(3):303-9. doi: 10.1016/j.femsim.2005.01.004.
A hundred and six Pseudomonas aeruginosa isolates from clinical cases were screened using PCR for the presence of integrons and associated resistance gene cassettes. Forty-four isolates harboured class 1 integrons (41.5%), of which 29 isolates (66%) also carried gene cassettes. The aacA gene was most frequently found within class 1 integrons (69%), followed by blaOXA family genes (52%). From class 1 integron-positive strains, we detected a total of 15 isolates (34%) carrying no gene cassettes. Restriction fragment-length polymorphism analysis of the integrons variable region revealed some identical structures, as well as distinct profiles indicating heterogeneity among these cassette regions. Multiresistance was observed in 71% of isolates, nevertheless no strong correlation was observed between integron presence and multiresistance. This is the first report showing class 1 integron prevalence and gene cassette content in P. aeruginosa isolates from clinical settings in the Brazilian Amazon.
使用聚合酶链反应（PCR）对106株临床分离的铜绿假单胞菌进行筛选，以检测整合子及相关耐药基因盒的存在情况。44株分离菌携带1类整合子（41.5%），其中29株（66%）还携带基因盒。aacA基因在1类整合子中最常被发现（69%），其次是blaOXA家族基因（52%）。在1类整合子阳性菌株中，我们共检测到15株（34%）未携带基因盒。对整合子可变区的限制性片段长度多态性分析揭示了一些相同的结构，以及表明这些基因盒区域存在异质性的不同图谱。71%的分离菌观察到多重耐药，但整合子的存在与多重耐药之间未观察到强相关性。这是首份显示巴西亚马逊地区临床环境中铜绿假单胞菌分离株中1类整合子流行率及基因盒内容的报告。
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