线虫猪蛔虫线粒体tRNA中摆动修饰的异常用法。
Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum.
作者信息
Sakurai Masayuki, Ohtsuki Takashi, Suzuki Tsutomu, Watanabe Kimitsuna
机构信息
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.
出版信息
FEBS Lett. 2005 May 23;579(13):2767-72. doi: 10.1016/j.febslet.2005.04.009. Epub 2005 Apr 20.
To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm(5)U) was identified at the wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln), while 5-carboxymethylaminomethyl-2-thiouridine (cmnm(5)s(2)U) was present in tRNA(UAA)(Leu)andtRNA(Trp). In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm(5)s(2)U is used in tRNA(UAA)(Leu)andtRNA(Trp). The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.
为了解具有异常二级结构的线虫线粒体tRNA的解码特性，分析了猪蛔虫线粒体tRNA对应以嘌呤结尾的双密码子家族的摆动位置上的转录后修饰。在tRNA（Lys）、tRNA（Glu）和tRNA（Gln）的摆动位置鉴定出5-羧甲基氨基甲基尿苷（cmnm（5）U），而5-羧甲基氨基甲基-2-硫尿苷（cmnm（5）s（2）U）存在于tRNA（UAA）（Leu）和tRNA（Trp）中。在大多数细菌和线粒体tRNA中，2-硫尿苷衍生物存在于Lys、Glu和Gln的tRNA中。没有关于cmnm（5）s（2）U用于tRNA（UAA）（Leu）和tRNA（Trp）的报道。摆动修饰的异常使用可能有助于线虫线粒体mRNA的解码。
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