Marakhova Irina, Karitskaya Inna, Aksenov Nikolay, Zenin Valerii, Vinogradova Tatjana
Institute of Cytology RAS, St. Petersburg, Russia.
FEBS Lett. 2005 May 23;579(13):2773-80. doi: 10.1016/j.febslet.2005.03.096. Epub 2005 Apr 20.
The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T cells, IL-2 increases both the transport activity of Na/K pump and the content of Na,K-ATPase alpha1 protein in a time-dependent manner. A correlation was found between the long-term elevation in ouabain-sensitive Rb influxes and the increase in alpha1 protein content in late activated T cells. These results suggest that (1) the increased expression of Na,K-ATPase proteins underlie the cell cycle-dependent upregulation of ion pump during T cell transformation, and (2) IL-2 is involved in the regulated expression of Na,K-ATPase in human lymphocytes.
本研究首次证明，在T细胞母细胞转化过程中，钠钾ATP酶催化性α1亚基的丰度增加。抗增殖剂量的免疫抑制剂环孢素A可减少活化淋巴细胞中α1蛋白的诱导表达。此外，在有功能的T细胞中，白细胞介素-2能以时间依赖性方式增加钠钾泵的转运活性和钠钾ATP酶α1蛋白的含量。在晚期活化T细胞中，哇巴因敏感性铷内流的长期升高与α1蛋白含量的增加之间存在相关性。这些结果表明：（1）钠钾ATP酶蛋白表达增加是T细胞转化过程中离子泵细胞周期依赖性上调的基础；（2）白细胞介素-2参与了人淋巴细胞中钠钾ATP酶的调节性表达。
