Marakhova I I, Vinogradova T A, Toropova F V
Tsitologiia. 1995;37(12):1167-79.
A transition of phytohemagglutinin (PHA)-activated human lymphocytes from resting state to proliferation is accompanied by an early and a long-term, delayed increase in ouabain-sensitive Rb influx. The long-term (between 16 and 48 h of PHA action) activation of the Na,K-ATPase pump is concomitant with the enlargement of lymphocytes and precedes the onset of DNA synthesis. When inhibiting the protein synthesis in activated lymphocytes, cycloheximide fails to suppress the early rise in Rb influx; however, it abolishes completely the increase in ouabain-sensitive Rb fluxes after 2 h of PHA stimulation. The inhibitors of transcription, actinomycin D and alpha-amantin, in doses suppressing the increase in the PHA-induced RNA synthesis, do not abolish the elevated Rb influx within 20-24 h of PHA action, and inhibit a growth-related increase in Rb influx during the 2nd day of activation. It is concluded that in mitogen-stimulated human lymphocytes the protein synthesis dependent enhancement of Na,K-ATPase pump activity is different in its nature at different phases of G0/G1/S progression. Unlike the early pump stimulation (0.5-2 h), the late elevation of K influxes associated with the growth of lymphocytes, is due to the regulation of the Na,K-ATPase pump at translational (2-20 h) and transcriptional (after 20 h) levels.
植物血凝素（PHA）激活的人淋巴细胞从静止状态向增殖状态的转变伴随着哇巴因敏感的铷流入的早期和长期延迟增加。Na，K - ATP酶泵的长期激活（PHA作用16至48小时之间）与淋巴细胞的增大同时发生，并先于DNA合成的开始。当抑制活化淋巴细胞中的蛋白质合成时，放线菌酮不能抑制铷流入的早期升高；然而，它完全消除了PHA刺激2小时后哇巴因敏感的铷通量的增加。转录抑制剂放线菌素D和α-鹅膏蕈碱，在抑制PHA诱导的RNA合成增加的剂量下，在PHA作用20 - 24小时内不会消除升高的铷流入，并且在激活的第二天抑制与生长相关的铷流入增加。结论是，在有丝分裂原刺激的人淋巴细胞中，可以看出，在G0/G1/S进展的不同阶段，依赖蛋白质合成的Na，K - ATP酶泵活性增强的性质不同。与早期泵刺激（0.5 - 2小时）不同，与淋巴细胞生长相关的后期钾流入升高是由于在翻译水平（2 - 20小时）和转录水平（20小时后）对Na，K - ATP酶泵的调节。
