采用高效液相色谱-紫外检测法同时测定三七中六种主要活性皂苷的含量。
Simultaneous quantification of six major active saponins of Panax notoginseng by high-performance liquid chromatography-UV method.
作者信息
Li Lie, Zhang Jin-Lan, Sheng Yu-Xin, Guo De-An, Wang Qiao, Guo Hong-Zhu
机构信息
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, No. 38 Xueyuan Road, Beijing 100083, PR China.
出版信息
J Pharm Biomed Anal. 2005 Jun 1;38(1):45-51. doi: 10.1016/j.jpba.2004.12.002.
A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed for the first time to simultaneously quantify the six major active saponins of Panax notoginseng, namely notoginsenoside R1, ginsenoside Rg1, Rb1, Rg2, Rh1 and Rd. Astragaloside IV is used as the internal standard. This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0.01% formic acid in 30 min. The method provided good reproducibility and sensitivity for the quantification of six saponins with overall intra- and inter-day precision and accuracy of less than 4.0% and higher than 90%, respectively. This assay is successfully applied to the determination of the six saponins in 23 notoginseng samples. The results indicated that the developed HPLC assay can be readily utilized as a quality control method for P. notoginseng.
首次开发了一种简单、灵敏且特异的高效液相色谱 - 紫外检测法（HPLC - UV），用于同时定量三七的六种主要活性皂苷，即三七皂苷R1、人参皂苷Rg1、Rb1、Rg2、Rh1和Rd。黄芪甲苷用作内标。该HPLC分析在反相C18柱上进行，乙腈和0.01%甲酸在30分钟内进行梯度洗脱。该方法对六种皂苷的定量具有良好的重现性和灵敏度，日内和日间精密度和准确度总体分别小于4.0%和高于90%。该分析方法成功应用于23个三七样品中六种皂苷的测定。结果表明，所开发的HPLC分析方法可 readily 用作三七的质量控制方法。 （注：“readily”此处翻译为“容易地、 readily 地”，结合语境推测是“易于、 readily 地”意思，但原英文表述可能有误，正常应该是“readily”）
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