Bonfill Mercè, Casals Isidre, Palazón Javier, Mallol Anna, Morales Carmen
Universitat de Barcelona, Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Avda Diagonal no. 643, 08028 Barcelona, Catalonia, Spain.
Biomed Chromatogr. 2002 Feb;16(1):68-72. doi: 10.1002/bmc.120.
A reversed-phase high-performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb(1), Rb(2), Rc, Rd, Rg(1), Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 x 4 mm Lichrospher, 5 microm, 100 A diol column with detection at 203 nm. The method was validated over the range of 2.5-20 ng/microL using a 20 microL sample volume. The average accuracy at five concentrations was 90-100%, and the within-day and between-day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively.
本文描述了一种反相高效液相色谱法，用于同时定量测定药物制剂中的七种人参皂苷，即Rb(1)、Rb(2)、Rc、Rd、Rg(1)、Re和Rf。使用250×4 mm、5μm、100 Å二醇柱，在203 nm处进行检测，不到20分钟即可实现色谱分离。该方法使用20 μL进样体积，在2.5 - 20 ng/μL范围内进行了验证。五个浓度水平下的平均准确度为90 - 100%，日内和日间精密度以变异系数表示，范围为1%至7%。该方法对每种人参皂苷的检测限和定量限分别为进样20 ng和50 ng。
