用单一对照品同时测定草药中的多种人参皂苷。
Simultaneous Determination of Multiple Ginsenosides in Herbal Medicines with One Single Reference Standard.
作者信息
Wu Chunwei, Guan Qingxiao, Wang Shumei, Rong Yueying
机构信息
Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, P.R. China.
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine, Guangzhou 510006, P.R. China.
出版信息
Pharmacogn Mag. 2017 Jan;13(Suppl 1):S84-S89. doi: 10.4103/pm.pm_274_16. Epub 2017 Apr 7.
BACKGROUND
Root of C. A. Mey (Renseng in Chinese) is a famous Traditional Chinese Medicine. Ginsenosides are the major bioactive components. However, the shortage and high cost of some ginsenoside reference standards make it is difficult for quality control of .
OBJECTIVE
A method, single standard for determination of multicomponents (SSDMC), was developed for the simultaneous determination of nine ginsenosides in (ginsenoside Rg, Re, Rf, Rg, Rb, Rc, Rb, Rb, Rd).
MATERIALS AND METHODS
The analytes were separated on Inertsil ODS-3 C18 (250 mm × 4.6 mm, 5 μm) with gradient elution of acetonitrile and water. The flow rate was 1 mL/min and detection wavelength was set at 203 nm. The feasibility and accuracy of SSDMC were checked by the external standard method, and various high-performance liquid chromatographic (HPLC) instruments and chromatographic conditions were investigated to verify its applicability. Using ginsenoside Rg as the internal reference substance, the contents of other eight ginsenosides were calculated according to conversion factors (F) by HPLC.
RESULTS
The method was validated with linearity ( ≥ 0.9990), precision (relative standard deviation [RSD] ≤2.9%), accuracy (97.5%-100.8%, RSD ≤ 1.6%), repeatability, and stability. There was no significant difference between the SSDMC method and the external standard method.
CONCLUSION
New SSDMC method could be considered as an ideal mean to analyze the components for which reference standards are not readily available.
SUMMARY
A method, single standard for determination of multicomponents (SSDMC), was established by high-performance liquid chromatography for the simultaneous determination of nine ginsenosides in (ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3, Rd)Various chromatographic conditions were investigated to verify applicability of FsThe feasibility and accuracy of SSDMC were checked by the external standard method. DRT: Different value of retention time; F: Conversion factor; HPLC: High-performance Liquid Chromatography; LOD: Limit of detection; LOQ: Limit of quantitation; PD: Percent difference; PPD: 20(S)-protopanaxadiol; PPT: 20(S)-protopanaxatriol; RSD: Relative standard deviation; SSDMC: Single Standard for Determination of Multicomponents; TCM: Traditional Chinese Medicine.
背景
人参是一种著名的传统中药。人参皂苷是其主要生物活性成分。然而，一些人参皂苷对照品短缺且成本高昂，这使得人参的质量控制变得困难。
目的
建立一种多成分测定单标准法（SSDMC），用于同时测定人参中9种人参皂苷（人参皂苷Rg1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rb3、Rd）。
材料与方法
采用Inertsil ODS-3 C18色谱柱（250 mm×4.6 mm，5μm），以乙腈和水为流动相进行梯度洗脱分离分析物。流速为1 mL/min，检测波长设定为203 nm。采用外标法考察SSDMC的可行性和准确性，并研究了各种高效液相色谱（HPLC）仪器和色谱条件以验证其适用性。以人参皂苷Rg1为内参物，通过HPLC根据换算因子（F）计算其他8种人参皂苷的含量。
结果
该方法线性关系良好（r≥0.9990），精密度高（相对标准偏差[RSD]≤2.9%），准确度高（97.5%-100.8%，RSD≤1.6%），具有良好的重复性和稳定性。SSDMC法与外标法之间无显著差异。
结论
新的SSDMC法可被视为分析缺乏对照品的成分的理想方法。
总结
采用高效液相色谱法建立了一种多成分测定单标准法（SSDMC），用于同时测定人参中9种人参皂苷（人参皂苷Rg1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rb3、Rd）。研究了各种色谱条件以验证换算因子（F）的适用性。采用外标法考察SSDMC的可行性和准确性。DRT：保留时间不同值；F：换算因子；HPLC：高效液相色谱；LOD：检测限；LOQ：定量限；PD：百分差异；PPD：20（S）-原人参二醇；PPT：20（S）-原人参三醇；RSD：相对标准偏差；SSDMC：多成分测定单标准法；TCM：传统中药
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