Bruckner-Lea C, Durney C H, Janata J, Rappaport C, Kaminski M
Electrical Engineering Department, University of Utah, Salt Lake City 84112.
Bioelectromagnetics. 1992;13(2):147-62. doi: 10.1002/bem.2250130208.
The possibility that weak, ac and dc magnetic fields in combination may affect binding equilibria of calcium-ions (Ca2+) was investigated with two metallochromic dyes as calcium-binding molecules: murexide and arsenazo III. Calcium-dye equilibria were followed by measuring solution absorbances with a fiber-optic spectrophotometer. A Ca(2+)-arsenazo solution was also used indirectly to monitor the binding of Ca2+ to calmodulin. Parallel, ac and dc magnetic fields were applied to each preparation. The ac magnetic field was held constant during each of a series of experiments at a frequency in the range between 50 and 120 Hz (sine wave) or at 50 pps (square wave) and at an rms flux density in the range between 65 and 156 microT. The dc magnetic field was then varied from 0 to 299 microT at 1.3 microT increments. The magnetic fields did not measurably affect equilibria in the binding of metallochromic dyes or calmodulin to Ca2+.
利用两种金属络合染料（紫脲酸铵和偶氮胂III）作为钙离子结合分子，研究了弱交变磁场和直流磁场共同作用可能影响钙离子（Ca2+）结合平衡的可能性。通过使用光纤分光光度计测量溶液吸光度来跟踪钙-染料平衡。还间接使用Ca(2+)-偶氮胂溶液来监测Ca2+与钙调蛋白的结合。对每个制剂施加平行的交变磁场和直流磁场。在一系列实验的每一次实验中，交变磁场在50至120赫兹（正弦波）范围内的频率或50次/秒（方波）下保持恒定，均方根磁通密度在65至156微特斯拉范围内。然后，直流磁场以1.3微特斯拉的增量从0变化到299微特斯拉。磁场对金属络合染料或钙调蛋白与Ca2+的结合平衡没有可测量的影响。
