Hendee S P, Faour F A, Christensen D A, Patrick B, Durney C H, Blumenthal D K
Department of Bioengineering, University of Utah, Salt Lake City 84112, USA.
Biophys J. 1996 Jun;70(6):2915-23. doi: 10.1016/S0006-3495(96)79861-2.
Mechanisms by which weak electromagnetic fields may affect biological systems are of current interest because of their potential health effects. Lednev has proposed an ion parametric resonance hypothesis (Lednev, 1991, Bioelectromagnetics, 12:71-75), which predicts that when the ac, frequency of a combined dc-ac magnetic field equals the cyclotron frequency of calcium, the affinity of calcium for calcium-binding proteins such as calmodulin will be markedly affected. The present study evaluated Lednev's theory using two independent systems, each sensitive to changes in the affinity of calcium for calmodulin. One of the systems used was the calcium/calmodulin-dependent activation of myosin light chain kinase, a system similar to that previously used by Lednev. The other system monitored optical changes in the binding of a fluorescent peptide to the calcium/calmodulin complex. Each system was exposed to a 20.9 microT static field superimposed on a 20.9 microT sinusoidal field over a narrow frequency range centered at 16 Hz, the cyclotron frequency of the unhydrated calcium ion. In contrast to Lednev's predictions, no significant effect of combined dc-ac magnetic fields on calcium/calmodulin interactions was indicated in either experimental system.
弱电磁场可能影响生物系统的机制因其潜在的健康影响而受到当前关注。列德涅夫提出了一种离子参数共振假说（列德涅夫，1991年，《生物电磁学》，12:71 - 75），该假说预测，当直流 - 交流磁场的交流频率等于钙的回旋频率时，钙对钙结合蛋白（如钙调蛋白）的亲和力将受到显著影响。本研究使用两个独立的系统对列德涅夫的理论进行了评估，每个系统都对钙对钙调蛋白亲和力的变化敏感。所使用的系统之一是肌球蛋白轻链激酶的钙/钙调蛋白依赖性激活，这是一个与列德涅夫之前使用的系统类似的系统。另一个系统监测荧光肽与钙/钙调蛋白复合物结合时的光学变化。每个系统都暴露在一个叠加在20.9微特斯拉正弦波场上的20.9微特斯拉静磁场中，频率范围狭窄，以16赫兹为中心，即无水钙离子的回旋频率。与列德涅夫的预测相反，在任何一个实验系统中，直流 - 交流磁场对钙/钙调蛋白相互作用均未显示出显著影响。
