Jones R A, Sheppard A R
Research Service-151, J.L. Pettis Memorial VA Medical Center, Loma Linda, California 92357.
Bioelectromagnetics. 1992;13(3):199-207. doi: 10.1002/bem.2250130304.
Long-term studies of the effects of low-frequency magnetic fields on cells in culture require an incubator that is free of contaminating magnetic fields and that provides automatic control of exposure duration, uniform applied fields, a uniform and well-controlled environmental temperature, and high reliability of the total system. We describe a dual-incubator system in which the enclosed solenoid of either unit can provide a sham exposure while the other generates a magnetic induction of up to 500 microT. Each solenoid-incubator unit contains an enclosed rack that can hold as many as 140 petri dishes for culturing cells; each unit is heated radiantly by circulating warm water. Field-exposure conditions and temperatures are continuously monitored and controlled by a microcomputer.
对培养中的细胞进行低频磁场影响的长期研究需要一个没有污染磁场的培养箱，该培养箱要能自动控制暴露持续时间、提供均匀的施加磁场、保持均匀且可控的环境温度以及保证整个系统的高可靠性。我们描述了一种双培养箱系统，其中任何一个单元的封闭螺线管都可以提供假暴露，而另一个则可产生高达500微特斯拉的磁感应强度。每个螺线管培养箱单元都包含一个封闭的架子，可容纳多达140个用于培养细胞的培养皿；每个单元通过循环温水进行辐射加热。场暴露条件和温度由微型计算机持续监测和控制。
