Chaisavaneeyakorn Sujittra, Lucchi Naomi, Abramowsky Carlos, Othoro Caroline, Chaiyaroj Sansanee C, Shi Ya Ping, Nahlen Bernard L, Peterson David S, Moore Julie M, Udhayakumar Venkatachalam
Centers for Disease Control and Prevention, 4770 Buford Highway, Mail Stop F-12, Chamblee, GA 30341, USA.
Infect Immun. 2005 Jun;73(6):3287-93. doi: 10.1128/IAI.73.6.3287-3293.2005.
Previously, we have shown that macrophage migration inhibitory factor (MIF) was highly elevated in the placental intervillous blood (IVB) of Plasmodium falciparum-infected women. Here, we compared the expression of MIF in placental tissues obtained from P. falciparum-infected and -uninfected women. Immunoperoxidase staining showed a consistent pattern of MIF expression in syncytiotrophoblasts, extravillous trophoblasts, IVB mononuclear cells, and amniotic epithelial cells, irrespective of their malaria infection status. Cytotrophoblast, villous stroma, and Hofbauer cells showed focal staining. Only amniotic epithelial and IVB mononuclear cells from P. falciparum-infected placentas exhibited significantly higher level of MIF expression than uninfected placentas. Stimulation of syncytilized human trophoblast BeWo cells with P. falciparum-infected erythrocytes that were selected to bind these cells resulted in significant increases in MIF secretion, whereas control erythrocytes, lipopolysaccharides, and synthetic beta-hematin had minimal effect. These findings suggest that placental malaria modulates MIF expression in different placental compartments.
此前，我们已表明，在感染恶性疟原虫的女性的胎盘绒毛间隙血（IVB）中，巨噬细胞移动抑制因子（MIF）水平显著升高。在此，我们比较了从感染和未感染恶性疟原虫的女性获取的胎盘组织中MIF的表达情况。免疫过氧化物酶染色显示，无论疟原虫感染状态如何，合体滋养层细胞、绒毛外滋养层细胞、IVB单核细胞和羊膜上皮细胞中MIF的表达模式一致。细胞滋养层、绒毛间质和霍夫鲍尔细胞呈局灶性染色。仅来自感染恶性疟原虫胎盘的羊膜上皮细胞和IVB单核细胞的MIF表达水平显著高于未感染的胎盘。用经选择可与这些细胞结合的感染恶性疟原虫的红细胞刺激人合体滋养层BeWo细胞，可导致MIF分泌显著增加，而对照红细胞、脂多糖和合成β-血红素的影响极小。这些发现表明，胎盘疟疾可调节不同胎盘区室中MIF的表达。
