用高度纯化的结核分枝杆菌重组蛋白进行皮肤试验会在感染的豚鼠中引发结核菌素休克。
Skin test performed with highly purified Mycobacterium tuberculosis recombinant protein triggers tuberculin shock in infected guinea pigs.
作者信息
Reece Stephen T, Stride Nicole, Ovendale Pamela, Reed Steven G, Campos-Neto Antonio
机构信息
The Forsyth Institute, 140 The Fenway, Boston, MA 02115, USA.
出版信息
Infect Immun. 2005 Jun;73(6):3301-6. doi: 10.1128/IAI.73.6.3301-3306.2005.
Abstract
Tuberculin shock due to inoculation of Mycobacterium tuberculosis antigens in patients with tuberculosis is a serious syndrome originally described over 100 years ago by Robert Koch. Here, we present experimental evidence that a single M. tuberculosis recombinant protein, CFP-10, triggers this syndrome. Intradermal inoculation of CFP-10 elicits in M. tuberculosis-infected mice high levels of serum tumor necrosis factor alpha and causes tuberculin shock in infected guinea pigs characterized by hypothermia and death within 6 to 48 h after the antigen inoculation. Autopsies of these animals revealed intense polycythemia and hemorrhagic patches in the lung parenchyma, a pathological observation consistent with tuberculin shock. These results point to the possible occurrence of tuberculin shock in sensitive individuals inoculated with highly purified M. tuberculosis recombinant proteins as vaccine candidates or skin test reagents.
摘要
在结核病患者中，因接种结核分枝杆菌抗原而引发的结核菌素休克是一种严重的综合征，早在100多年前就由罗伯特·科赫首次描述。在此，我们提供实验证据表明，单一的结核分枝杆菌重组蛋白CFP-10可引发该综合征。对感染结核分枝杆菌的小鼠进行CFP-10皮内接种，可使其血清肿瘤坏死因子α水平升高，并在感染的豚鼠中引发结核菌素休克，其特征为体温过低，并在抗原接种后6至48小时内死亡。对这些动物进行尸检发现，肺实质出现严重的红细胞增多症和出血斑，这一病理观察结果与结核菌素休克一致。这些结果表明，在接种高度纯化的结核分枝杆菌重组蛋白作为候选疫苗或皮肤试验试剂的敏感个体中，可能会发生结核菌素休克。
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Clin Infect Dis. 2004 Aug 1;39(3):295-9. doi: 10.1086/421494. Epub 2004 Jul 16.
2
Differential immune responses and protective efficacy induced by components of a tuberculosis polyprotein vaccine, Mtb72F, delivered as naked DNA or recombinant protein.结核多蛋白疫苗Mtb72F的组分以裸DNA或重组蛋白形式递送时所诱导的差异性免疫反应和保护效力。
J Immunol. 2004 Jun 15;172(12):7618-28. doi: 10.4049/jimmunol.172.12.7618.
3
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Mol Microbiol. 2004 Jan;51(2):359-70. doi: 10.1046/j.1365-2958.2003.03844.x.
4
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