Wilson Iain W, Kennedy Gavin C, Peacock James W, Dennis Elizabeth S
CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.
Plant Cell Physiol. 2005 Aug;46(8):1190-201. doi: 10.1093/pcp/pci128. Epub 2005 May 20.
The transition to flowering occurs at the shoot apex; however, most of the characterized genes that affect the timing of floral induction are expressed throughout the plant. To further our understanding of these genes and the flowering process, the vegetative molecular phenotypes of 16 Arabidopsis mutants associated with the major flowering initiation pathways were assayed using a 13,000 clone microarray under two different conditions that affect flowering. All mutants showed at least one change in gene expression other than the mutant flowering gene. Metabolism- and defence-related pathways were the areas with the most frequent gene expression changes detected in the mutants. Several genes such as EARLI1 were differentially expressed in a number of flowering mutants from different flowering pathways. Analysis of the promoter regions of genes differentially expressed identified common promoter elements, indicating some form of common regulation.
向开花的转变发生在茎尖；然而，大多数已鉴定的影响花诱导时间的基因在整个植株中都有表达。为了进一步了解这些基因和开花过程，我们使用一个包含13000个克隆的微阵列，在两种影响开花的不同条件下，对与主要开花起始途径相关的16个拟南芥突变体的营养分子表型进行了分析。所有突变体除了突变的开花基因外，至少在基因表达上有一个变化。代谢和防御相关途径是在突变体中检测到基因表达变化最频繁的领域。一些基因，如EARLI1，在来自不同开花途径的多个开花突变体中差异表达。对差异表达基因的启动子区域进行分析，鉴定出了共同的启动子元件，这表明存在某种形式的共同调控。
