Lay Fung T, Mills Grant D, Hulett Mark D, Kvansakul Marc
Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):85-8. doi: 10.1107/S1744309111049530. Epub 2011 Dec 24.
Plant defensins are small (~5 kDa) basic cysteine-rich proteins that are being explored in important agricultural crops for their ability to confer enhanced disease resistance against fungal pathogens. NaD1, isolated from the flowers of the ornamental tobacco (Nicotiana alata), is a particularly well characterized antifungal defensin. Here, the crystallization and preliminary X-ray crystallographic analysis of NaD1 is reported. Crystals of NaD1 were crystallized using the sitting-drop vapour-diffusion method at 291 K. Data were collected from two crystal forms to 1.4 and 1.6 Å resolution, respectively. The crystals of form A belonged to the monoclinic space group P2(1), with unit-cell parameters a = 32.697, b = 32.685, c = 41.977 Å, α = 90, β = 100.828, γ = 90°, whereas crystals of form B belonged to the trigonal space group P3(2)21, with unit-cell parameters a = b = 33.091, c = 128.77 Å, α = β = 90, γ = 120°.
植物防御素是一类小的（约5 kDa）富含碱性半胱氨酸的蛋白质，因其能够增强对真菌病原体的抗病能力而正在重要农作物中进行研究。从观赏烟草（Nicotiana alata）花朵中分离得到的NaD1是一种特征特别明确的抗真菌防御素。本文报道了NaD1的结晶及初步X射线晶体学分析。NaD1晶体采用坐滴气相扩散法于291 K下结晶。分别从两种晶体形式收集数据至1.4 Å和1.6 Å分辨率。A晶型晶体属于单斜空间群P2(1)，晶胞参数a = 32.697，b = 32.685，c = 41.977 Å，α = 90，β = 100.828，γ = 90°；而B晶型晶体属于三方空间群P3(2)21，晶胞参数a = b = 33.091，c = 128.77 Å，α = β = 90，γ = 120°。
