Silver H M, Siler-Khodr T, Prihoda T J, Gibbs R S
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio.
Am J Perinatol. 1992 Mar;9(2):69-74. doi: 10.1055/s-2007-994674.
In studying the effects of amniotic fluid on bacterial growth in a laboratory model, we noted that the pH of the fluid appeared to exert an independent effect. This study was designed to test the ability to control the growth of Escherichia coli in amniotic fluid simply by controlling two important growth conditions, pH and osmolality. The effects of pH and osmolality on growth of E. coli were systematically studied in a standard media and in amniotic fluid. Optimal ranges in standard media were pH of 5.6 to 6.6 and osmolality of 150 to 215 mOsm. When the results of growth at 24 hours were corrected for pH by analysis of covariance, the presence of amniotic fluid or phosphate had no effect. We found pH to be the only variable predictive of bacterial growth in amniotic fluid in this laboratory model.
在实验室模型中研究羊水对细菌生长的影响时，我们注意到羊水的pH值似乎发挥着独立作用。本研究旨在测试仅通过控制两个重要的生长条件——pH值和渗透压，能否控制大肠杆菌在羊水中的生长。在标准培养基和羊水中系统地研究了pH值和渗透压对大肠杆菌生长的影响。标准培养基中的最佳范围是pH值为5.6至6.6，渗透压为150至215毫摩尔渗透压浓度。通过协方差分析对24小时生长结果进行pH值校正后，羊水或磷酸盐的存在没有影响。我们发现在这个实验室模型中，pH值是预测羊水中细菌生长的唯一变量。
