Phan T T, Lim I J, Tan E K, Bay B H, Lee S T
Department of Plastic Surgery, Singapore General Hospital, Republic of Singapore.
Cell Tissue Bank. 2005;6(2):91-7. doi: 10.1007/s10561-004-3904-8.
The use of polymer-based delivery systems, on which cells are cultured and transferred, improves the ease of handling and transfer of the keratinocytes. A transparent polymer also allows observation of cell growth prior to grafting as well as re-epithelialization after grafting to the wound. We have developed techniques for cultured keratinocytes on Tegaderm (3M), an inexpensive and easily available polyurethane-based wound dressing, for treatment of burn and chronic wounds. In this study, we evaluate cell culture characteristics of three different cell types, human epidermal keratinocytes, human dermal fibroblasts and pig bone marrow mesenchymal stem cells on Tegaderm membrane.
Cells were isolated from human skin or pig bone marrow and cultured on membranes for a period of five days. Cell proliferation was assessed by colorimetric assay (MTT) and scanning electron microscopy.
This study confirms that Tegaderm membranes support attachment and growths for these cell types, with those growth characteristics are similar, if not as good as that of optimal condition of tissue culture plastics. Data from our study suggest that Tegaderm membranes can be used, modified and developed further as an economical and easily available material for tissue engineered skin.
基于聚合物的递送系统用于培养和转移细胞，提高了角质形成细胞处理和转移的便利性。透明聚合物还能在移植前观察细胞生长情况，以及在移植到伤口后观察再上皮化过程。我们已开发出在得佳贴（3M公司）上培养角质形成细胞的技术，得佳贴是一种价格低廉且易于获得的聚氨酯基伤口敷料，用于治疗烧伤和慢性伤口。在本研究中，我们评估了三种不同细胞类型，即人表皮角质形成细胞、人真皮成纤维细胞和猪骨髓间充质干细胞在得佳贴膜上的细胞培养特性。
从人皮肤或猪骨髓中分离细胞，并在膜上培养五天。通过比色法（MTT）和扫描电子显微镜评估细胞增殖情况。
本研究证实得佳贴膜支持这些细胞类型的附着和生长，其生长特性相似，即便不如组织培养塑料的最佳条件那么好。我们的研究数据表明，得佳贴膜可作为一种经济且易于获得的材料，进一步用于组织工程皮肤的制备、改良和开发。
