Venugopal Jayarama Reddy, Zhang Yanzhong, Ramakrishna Seeram
Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Singapore.
Artif Organs. 2006 Jun;30(6):440-6. doi: 10.1111/j.1525-1594.2006.00239.x.
Novel cost-effective electrospun nanofibrous membrane is established for wound dressing and allogeneic cultured dermal substitute through the cultivation of human dermal fibroblast for skin defects. Synthetic polymers are generally used for tissue engineering and drug delivery applications because of their remarkable mechanical stability and slow degradation. Polycaprolactone (PCL) is used as a bioresorbable polymer in numerous medical devices as well as for tissue engineering applications. The large surface area of the polymer nanofibers with specific modifications facilitates cell adhesion and control of their cellular functions. The objectives of this study was to fabricate electrospun nanofibrous membrane from biodegradable PCL for wound dressing and collagen-blended nanofibrous membrane, and to examine fibroblast attachment, cell proliferation, and morphology of cell matrix interaction. Results of the present investigation prove that the porous nanofibrous membrane is suitable for wound dressing and modified PCL-blended collagen nanofibrous membrane is suitable for the attachment and proliferation of fibroblast, and might have the potential to be applied in tissue engineering as a dermal substitute for the treatment of skin defects and burn wounds.
通过培养人真皮成纤维细胞用于皮肤缺损，制备了新型具有成本效益的电纺纳米纤维膜用于伤口敷料和同种异体培养真皮替代物。合成聚合物因其卓越的机械稳定性和缓慢降解性，通常用于组织工程和药物递送应用。聚己内酯（PCL）在众多医疗设备以及组织工程应用中用作生物可吸收聚合物。经过特定改性的聚合物纳米纤维的大表面积有助于细胞黏附并控制其细胞功能。本研究的目的是用可生物降解的PCL制备用于伤口敷料的电纺纳米纤维膜以及胶原共混纳米纤维膜，并研究成纤维细胞的附着、细胞增殖以及细胞与基质相互作用的形态。本研究结果证明，多孔纳米纤维膜适用于伤口敷料，而改性PCL共混胶原纳米纤维膜适用于成纤维细胞的附着和增殖，并且可能有潜力作为真皮替代物应用于组织工程，用于治疗皮肤缺损和烧伤创面。
